• Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (PubMed=6305508).
  

PubMed=6305508; DOI=10.1016/0092-8674(83)90422-1
Urlaub G., Kas E., Carothers A.M., Chasin L.A.
Deletion of the diploid dihydrofolate reductase locus from cultured mammalian cells.
Cell 33:405-412(1983)

PubMed=16403443; DOI=10.1016/j.bbrc.2005.12.111
Derouazi M., Martinet D., Besuchet-Schmutz N., Flaction R., Wicht M., Bertschinger M., Hacker D.L., Beckmann J.S., Wurm F.M.
Genetic characterization of CHO production host DG44 and derivative recombinant cell lines.
Biochem. Biophys. Res. Commun. 340:1069-1077(2006)

DOI=10.1007/978-1-4020-5476-1_59
Martinet D., Derouazi M., Besuchet-Schmutz N., Wicht M., Beckmann J.S., Wurm F.M.
Karyotype of CHO DG44 cells.
(In book chapter) Cell technology for cell products. Proceedings of the 19th ESACT Meeting; Smith R. (eds.); pp.363-366; Springer; Dordrecht; Netherlands (2007)

PubMed=21345355; DOI=10.1016/j.jbiotec.2011.02.002
Florin L., Lipske C., Becker E., Kaufmann H.
Supplementation of serum free media with HT is not sufficient to restore growth properties of DHFR-/- cells in fed-batch processes -- implications for designing novel CHO-based expression platforms.
J. Biotechnol. 152:189-193(2011)

DOI=10.3390/pr1030296
Wurm F.M.
CHO quasispecies -- implications for manufacturing processes.
Processes 1:296-311(2013)

PubMed=23873082; DOI=10.1038/nbt.2624
Lewis N.E., Liu X., Li Y.-X., Nagarajan H., Yerganian G., O'Brien E., Bordbar A., Roth A.M., Rosenbloom J., Bian C., Xie M., Chen W.-B., Li N., Baycin-Hizal D., Latif H., Forster J., Betenbaugh M.J., Famili I., Xu X., Wang J., Palsson B.O.
Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome.
Nat. Biotechnol. 31:759-765(2013)

DOI=10.3390/pr5020020
Wurm F.M., Wurm M.J.
Cloning of CHO cells, productivity and genetic stability -- a discussion.
Processes 5:20.1-20.13(2017)

PubMed=28876938; DOI=10.1021/acs.jproteome.7b00382
Heffner K.M., Baycin-Hizal D., Yerganian G., Kumar A., Can O., O'Meally R.N., Cole R.N., Chaerkady R., Wu H., Bowen M.A., Betenbaugh M.J.
Lessons from the hamster: Cricetulus griseus tissue and CHO cell line proteome comparison.
J. Proteome Res. 16:3672-3687(2017)

PubMed=29987698; DOI=10.1007/s10616-018-0233-5; PMCID=PMC6214854
Kimura S., Omasa T.
Genome sequence comparison between Chinese hamster ovary (CHO) DG44 cells and mouse using end sequences of CHO BAC clones based on BAC-FISH results.
Cytotechnology 70:1399-1407(2018)

PubMed=32619503; DOI=10.1016/j.ymben.2020.06.002
Szeliova D., Ruckerbauer D.E., Galleguillos S.N., Petersen L.B., Natter K., Hanscho M., Troyer C., Causon T., Schoeny H., Christensen H.B., Lee D.-Y., Lewis N.E., Koellensperger G., Hann S., Nielsen L.K., Borth N., Zanghellini J.
What CHO is made of: variations in the biomass composition of Chinese hamster ovary cell lines.
Metab. Eng. 61:288-300(2020)

PubMed=32985598; DOI=10.1038/s41598-020-72959-8; PMCID=PMC7522264
Heffner K.M., Baycin-Hizal D., Majewska N.I., Kumar S., Dhara V.G., Zhu J., Bowen M.A., Hatton D., Yerganian G., Yerganian A., O'Meally R.N., Cole R.N., Betenbaugh M.J.
Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities.
Sci. Rep. 10:15841-15841(2020)

PubMed=34050613; DOI=10.1002/biot.202100165
Wurm M.J., Wurm F.M.
Naming CHO cells for bio-manufacturing: genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names.
Biotechnol. J. 16:e2100165.1-e2100165.13(2021)