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Cellosaurus Ms704 (CVCL_KA70)

[Text version]
Cell line name Ms704
Synonyms MS704
Accession CVCL_KA70
Resource Identification Initiative To cite this cell line use: Ms704 (RRID:CVCL_KA70)
Comments Characteristics: Useful for the production of fucose-negative antibodies.
Characteristics: Lacks both copies of the Dhfr gene.
Knockout cell: Method=Homologous recombination; UniProtKB; G3HCE4; Fut8.
Selected for resistance to: ChEBI; CHEBI:44185; Methotrexate (MTX).
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7180 (CHO-DG44)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=15352059; DOI=10.1002/bit.20151
Yamane-Ohnuki N., Kinoshita S., Inoue-Urakubo M., Kusunoki M., Iida S., Nakano R., Wakitani M., Niwa R., Sakurada M., Uchida K., Shitara K., Satoh M.
Establishment of FUT8 knockout Chinese hamster ovary cells: an ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity.
Biotechnol. Bioeng. 87:614-622(2004)

PubMed=16609957; DOI=10.1002/bit.20880
Kanda Y., Yamane-Ohnuki N., Sakai N., Yamano K., Nakano R., Inoue-Urakubo M., Misaka H., Iida S., Wakitani M., Konno Y., Yano K., Shitara K., Hosoi S., Satoh M.
Comparison of cell lines for stable production of fucose-negative antibodies with enhanced ADCC.
Biotechnol. Bioeng. 94:680-688(2006)

Cross-references
Encyclopedic resources Wikidata; Q54906807
Entry history
Entry creation22-Aug-2017
Last entry update30-Jan-2024
Version number9