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Cellosaurus Ms709 (CVCL_KA72)

[Text version]
Cell line name Ms709
Accession CVCL_KA72
Resource Identification Initiative To cite this cell line use: Ms709 (RRID:CVCL_KA72)
Comments Characteristics: Useful for the production of fucose-negative antibodies.
Knockout cell: Method=Homologous recombination; UniProtKB; G3HCE4; Fut8.
Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7180 (CHO-DG44)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=15352059; DOI=10.1002/bit.20151
Yamane-Ohnuki N., Kinoshita S., Inoue-Urakubo M., Kusunoki M., Iida S., Nakano R., Wakitani M., Niwa R., Sakurada M., Uchida K., Shitara K., Satoh M.
Establishment of FUT8 knockout Chinese hamster ovary cells: an ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity.
Biotechnol. Bioeng. 87:614-622(2004)

Cross-references
Encyclopedic resources Wikidata; Q54906810
Entry history
Entry creation22-Aug-2017
Last entry update19-Dec-2024
Version number10