Cellosaurus cell line CHOpper Standard (CVCL

Cellosaurus CHOpper Standard (CVCL_WB20)

Cross-references
Cell line name	CHOpper Standard
Accession	CVCL_WB20
Resource Identification Initiative	To cite this cell line use: CHOpper Standard (RRID:CVCL_WB20)
Comments	Group: Patented cell line. Registration: International Depositary Authority, Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ); ACC-2910. Doubling time: ~22 hours (Patent=US8962312). Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX). Genetic integration: Method=Transfection; Gene=UniProtKB; Q2MH30; Cricetulus griseus Dhfr. Transformant: NCIt; C129640; Cobalt-60 gamma radiation. Derived from site: In situ; Ovary; UBERON=UBERON_0000992. Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations	Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
Species of origin	Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy	Parent: CVCL_7180 (CHO-DG44)
Sex of cell	Female
Age at sampling	Adult
Category	Transformed cell line
Publications	PubMed=21345355; DOI=10.1016/j.jbiotec.2011.02.002 Florin L., Lipske C., Becker E., Kaufmann H. Supplementation of serum free media with HT is not sufficient to restore growth properties of DHFR-/- cells in fed-batch processes -- implications for designing novel CHO-based expression platforms. J. Biotechnol. 152:189-193(2011) Patent=US8962312 Kaufmann H., Florin L., Becker E., Studts J.M. Production host cell lines. Patent number US8962312, 24-Feb-2015
Encyclopedic resources	Wikidata; Q93453048
Entry history
Entry creation	24-May-2019
Last entry update	19-Dec-2024
Version number	9