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Cellosaurus CHO B8-300 (CVCL_VU03)

[Text version]
Cell line name CHO B8-300
Synonyms B8-300
Accession CVCL_VU03
Resource Identification Initiative To cite this cell line use: CHO B8-300 (RRID:CVCL_VU03)
Comments Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:3415; EPO.
Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7180 (CHO-DG44)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=3865178; DOI=10.1073/pnas.82.22.7580; PMCID=PMC391376
Lin F.-K., Suggs S.V., Lin C.-H., Browne J.K., Smalling R., Egrie J.C., Chen K.K., Fox G.M., Martin F., Stabinsky Z., Badrawi S.M., Lai P.-H., Goldwasser E.
Cloning and expression of the human erythropoietin gene.
Proc. Natl. Acad. Sci. U.S.A. 82:7580-7584(1985)

PubMed=2813359; DOI=10.1073/pnas.86.20.7819; PMCID=PMC298162
Takeuchi M., Inoue N., Strickland T.W., Kubota M., Wada M., Shimizu R., Hoshi S., Kozutsumi H., Takasaki S., Kobata A.
Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells.
Proc. Natl. Acad. Sci. U.S.A. 86:7819-7822(1989)

Cross-references
Encyclopedic resources Wikidata; Q93451114
Entry history
Entry creation24-May-2019
Last entry update19-Dec-2024
Version number8