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Cellosaurus CHO/CERT 2.20 (CVCL_B0VY)

[Text version]
Cell line name CHO/CERT 2.20
Accession CVCL_B0VY
Resource Identification Initiative To cite this cell line use: CHO/CERT 2.20 (RRID:CVCL_B0VY)
Comments Group: Patented cell line.
Registration: International Depositary Authority, Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ); ACC-2989.
Characteristics: Has improved secretion characteristics in comparison to non-transgenic cell lines.
Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:2205; CERT1 (Note=With p.Ser132Ala).
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7180 (CHO-DG44)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=19428735; DOI=10.1016/j.jbiotec.2009.02.014
Florin L., Pegel A., Becker E., Hausser A., Olayioye M.A., Kaufmann H.
Heterologous expression of the lipid transfer protein CERT increases therapeutic protein productivity of mammalian cells.
J. Biotechnol. 141:84-90(2009)

Patent=US9340592
Florin L., Becker E., Kaufmann H.
CHO/CERT cell lines.
Patent number US9340592, 17-May-2016

Cross-references
Encyclopedic resources Wikidata; Q108820040
Entry history
Entry creation23-Sep-2021
Last entry update19-Dec-2024
Version number7