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Cellosaurus Neuro-2a (CVCL_0470)

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Cell line name Neuro-2a
Synonyms NEURO-2A; Neuro 2a; Neuro2a; Neuro2A; N-2a; N2a; N2A; Nb2a; NB2a
Accession CVCL_0470
Resource Identification Initiative To cite this cell line use: Neuro-2a (RRID:CVCL_0470)
Comments Characteristics: Capable of differentiating into various types of neurons.
Doubling time: ~70 hours (DSMZ=ACC-148).
Omics: Deep proteome analysis.
Omics: SNP array analysis.
Miscellaneous: STR profile from personal communication of Rodriguez-Tarduchy Segovia, Gemma.
Derived from site: In situ; Peripheral nervous system; UBERON=UBERON_0000010.
Cell type: Neural crest derived neuroblast; CL=CL_0002676.
Disease Mouse neuroblastoma (NCIt: C21993)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: A/J.
Hierarchy Parent: CVCL_4343 (C-1300)
Children:
CVCL_B7VJ (Abcam Neuro-2a Arc KO)CVCL_6246 (AGN2a)CVCL_4510 (C1300 CLONE NA)
CVCL_C5RF (N2a #34)CVCL_C5RG (N2a #58)CVCL_ZM10 (N2A FLAG-Cdh1)
CVCL_A0TK (N2A Psen1/Psen2 dKO)CVCL_WI11 (N2a-R33)CVCL_WI08 (N2a/Gary)
CVCL_D649 (N2aEGFP)CVCL_D650 (N2aSW)CVCL_RW97 (Na 42/13)
CVCL_B436 (NB2a/d1)CVCL_DF49 (Neuro-2a NucLight Green)CVCL_DF50 (Neuro-2a NucLight Red)
CVCL_K046 (Neuro-2a-Luc)CVCL_C9FD (Neuro-2a/Cas9-Rosa26-Hyg)CVCL_C9FE (Neuro-2a/Cas9-Rosa26-Neo)
CVCL_C9FC (Neuro-2a/Cas9-Rosa26-Puro)CVCL_A9J5 (Neuro-2a/CMV-Luc)CVCL_C9FB (Neuro-2a/HF-Cas9-Rosa26)
CVCL_C9FF (Neuro-2a/LoxP-Cas9-hyg-Rosa26)CVCL_F275 (Neuro-2aTG)CVCL_D3XR (Neuro2a-RXR)
CVCL_E1GT (Neuro2A[TRb1])CVCL_6268 (ScN2a)CVCL_E0ZD (Ubigene N2A Aldh2 KO)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): Direct_author_submission

Markers:
Mouse STR 1-111
Mouse STR 1-217,18
Mouse STR 2-116
Mouse STR 3-212,13,14
Mouse STR 4-221.3,22.3
Mouse STR 5-515,17
Mouse STR 6-418,20
Mouse STR 6-712
Mouse STR 7-125.2
Mouse STR 8-116,17
Mouse STR 11-215
Mouse STR 12-116
Mouse STR 13-116.2,17.2
Mouse STR 15-321.3,22.3,23.3
Mouse STR 17-216
Mouse STR 18-322,23
Mouse STR 19-212
Mouse STR X-126,27

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/N2a_cell
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/n/cell-lines-detail-213.html
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/n/cell-lines-detail-384.html
https://www.researchgate.net/post/What_are_the_details_of_N2A_cell_differentiation_methods
Publications

PubMed=11470893; DOI=10.1073/pnas.151242598; PMCID=PMC55414
Enari M., Flechsig E., Weissmann C.
Scrapie prion protein accumulation by scrapie-infected neuroblastoma cells abrogated by exposure to a prion protein antibody.
Proc. Natl. Acad. Sci. U.S.A. 98:9295-9299(2001)

PubMed=14504404; DOI=10.1073/pnas.1834432100; PMCID=PMC208815
Klohn P.-C., Stoltze L., Flechsig E., Enari M., Weissmann C.
A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions.
Proc. Natl. Acad. Sci. U.S.A. 100:11666-11671(2003)

PubMed=19903493; DOI=10.1016/j.jneumeth.2009.11.004
Tremblay R.G., Sikorska M., Sandhu J.K., Lanthier P., Ribecco-Lutkiewicz M., Bani-Yaghoub M.
Differentiation of mouse Neuro 2A cells into dopamine neurons.
J. Neurosci. Methods 186:60-67(2010)

PubMed=25193168; DOI=10.1074/mcp.M113.037291; PMCID=PMC4256493
Hornburg D., Drepper C., Butter F., Meissner F., Sendtner M., Mann M.
Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics.
Mol. Cell. Proteomics 13:3410-3420(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. 3rd, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=25565633; DOI=10.1371/journal.pone.0117154; PMCID=PMC4286239
Oelschlegel A.M., Geissen M., Lenk M., Riebe R., Angermann M., Schaetzl H., Groschup M.H.
A bovine cell line that can be infected by natural sheep scrapie prions.
PLoS ONE 10:E0117154-E0117154(2015)

Cross-references
Cell line collections (Providers) Abcam; ab279975
ATCC; CCL-131
BCRC; 60026
BCRJ; 0189
CCLV; CCLV-RIE 0132
CCTCC; GDC0162
CLS; 400394
DSMZ; ACC-148
ECACC; 89121404
ICLC; ATL99007
IZSLER; BS TCL 128
JCRB; IFO50081
KCB; KCB 2014025YJ
RCB; RCB2639
TKG; TKG 0509
Cell line databases/resources CLO; CLO_0008154
CLO; CLO_0050180
MCCL; MCC:0000368
CLDB; cl3682
CLDB; cl3683
CLDB; cl3684
CLDB; cl3685
CLDB; cl4956
CLDB; cl5236
CCRID; 1101MOU-PUMC000291
CCRID; 3101MOUSCSP5035
CCRID; 3101MOUTCM29
CCRID; 4201MOU-CCTCC00162
DSMZCellDive; ACC-148
Lonza; 451
TOKU-E; 2746
TOKU-E; 3552
TOKU-E; 3563
Anatomy/cell type resources BTO; BTO_0001976
CRISP screens repositories BioGRID_ORCS_Cell_line; 1373
Chemistry resources ChEMBL-Cells; CHEMBL3307521
ChEMBL-Targets; CHEMBL614062
PubChem_Cell_line; CVCL_0470
Encyclopedic resources Wikidata; Q18391478
Experimental variables resources EFO; EFO_0022756
Proteomic databases PRIDE; PXD000065
PRIDE; PXD002290
PRIDE; PXD018875
Entry history
Entry creation04-Apr-2012
Last entry update19-Dec-2024
Version number34