Cellosaurus Neuro-2a/HF-Cas9-Rosa26 (CVCL_C9FB)
| Cell line name | Neuro-2a/HF-Cas9-Rosa26 |
|---|---|
| Synonyms | Neuro 2a/HF-Cas9-ROSA26 |
| Accession | CVCL_C9FB |
| Resource Identification Initiative | To cite this cell line use: Neuro-2a/HF-Cas9-Rosa26 (RRID:CVCL_C9FB) |
| Comments | Characteristics: Stably expresses a CMV-3xFLAG-HFCas9-NLS-bGHPolyA-EF1a-copGFP-T2A-HygR-SV40pa construct from the Rosa26 safe locus (GeneCopoeia=SL571). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asn497Ala, p.Arg661Ala, p.Gln695Ala and p.Gln926Ala = High-fidelity Cas9). Genetic integration: Method=Transfection/transduction; Gene=FPbase; 2BK6M; ppluGFP2 (copGFP, green fluorescent protein from P.plumata). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Derived from site: In situ; Peripheral nervous system; UBERON=UBERON_0000010. Cell type: Neural crest derived neuroblast; CL=CL_0002676. |
| Disease | Mouse neuroblastoma (NCIt: C21993) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: A/J. |
| Hierarchy | Parent: CVCL_0470 (Neuro-2a) |
| Sex of cell | Male |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | GeneCopoeia; SL571 |
| Encyclopedic resources | Wikidata; Q123033167 |
| Entry history | |
| Entry creation | 05-Oct-2023 |
| Last entry update | 10-Sep-2024 |
| Version number | 3 |