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Cellosaurus Neuro2a (HSE:EGFP) (CVCL_E6II)

[Text version]
Cell line name Neuro2a (HSE:EGFP)
Accession CVCL_E6II
Resource Identification Initiative To cite this cell line use: Neuro2a (HSE:EGFP) (RRID:CVCL_E6II)
Comments Characteristics: Constitutively expresses mCherry and expresses EGFP upon induction by stress. Transfected with two constructs, pCMV-mCherry and pminHsp70p-EGFP where EGFP is under the control of a minimal Hsp70 promoter (PubMed=32661089).
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Peripheral nervous system; UBERON=UBERON_0000010.
Cell type: Neural crest derived neuroblast; CL=CL_0002676.
Disease Mouse neuroblastoma (NCIt: C21993)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: A/J.
Hierarchy Parent: CVCL_0470 (Neuro-2a)
Sex of cell Male
Category Cancer cell line
Publications

PubMed=32661089; DOI=10.1242/jcs.243709
San Gil R., Cox D., McAlary L., Berg T., Walker A.K., Yerbury J.J., Ooi L., Ecroyd H.
Neurodegenerative disease-associated protein aggregates are poor inducers of the heat shock response in neuronal cells.
J. Cell Sci. 133:jcs243709.1-jcs243709.15(2020)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1