Cellosaurus logo
expasy logo

Cellosaurus MDCK (CVCL_0422)

[Text version]
Cell line name MDCK
Synonyms MDCK (NBL-2); MDCK(NBL-2); NBL-2; Madin-Darby Canine Kidney; Madin Darby Canine Kidney
Accession CVCL_0422
Resource Identification Initiative To cite this cell line use: MDCK (RRID:CVCL_0422)
Comments Group: Vaccine production cell line.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Characteristics: Used for virus production for vaccine development, toxicology and industrial biotechnology research, and high-throughput screening (ATCC=CCL-34).
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Omics: Transcriptome analysis by RNAseq.
Caution: There seem to be two distinct cell lines which were assigned NCBI_Iran catalog number C426.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
Hierarchy Children:
CVCL_4164 (DoCl1 (S+L-))CVCL_WL43 (hCK)CVCL_YJ87 (LINTERNA MDCK)
CVCL_GY95 (Luc9.1)CVCL_2A36 (MDCK 33016)CVCL_DF69 (MDCK 9B9-1B1)
CVCL_DF70 (MDCK 9B9-1E4)CVCL_ZV91 (MDCK Actin-cpstFRET-Actin)CVCL_ZV92 (MDCK Actinin-C-cpstFRET)
CVCL_ZV93 (MDCK Actinin-M-cpstFRET)CVCL_D5IF (MDCK BLVRA KO)CVCL_C8RJ (MDCK ConAr)
CVCL_ZV90 (MDCK cpstFRET-Actin)CVCL_C8RK (MDCK RCAr)CVCL_D3W3 (MDCK siat7e-expressing clone 1)
CVCL_D3W4 (MDCK siat7e-expressing clone 2)CVCL_AP67 (MDCK Sky1023)CVCL_AP68 (MDCK Sky10234)
CVCL_AP69 (MDCK Sky3851)CVCL_V359 (MDCK Tet-Off)CVCL_B5WN (MDCK tGFP-APP)
CVCL_B0N7 (MDCK-15D3)CVCL_B4IA (MDCK-2B2)CVCL_WL59 (MDCK-6A8)
CVCL_K177 (MDCK-AD)CVCL_AP66 (MDCK-B-702)CVCL_A2JC (MDCK-C09)
CVCL_AP70 (MDCK-C11)CVCL_A2JD (MDCK-C35)CVCL_0423 (MDCK-C7)
CVCL_DF31 (MDCK-chAbcb1)CVCL_D5I6 (MDCK-dADAM17)CVCL_D5HY (MDCK-dEGF_#1)
CVCL_D5I7 (MDCK-dEGF_#2)CVCL_D5I5 (MDCK-dEGF/HBEGF/TGFalpha/EREG KO 1)CVCL_D5HZ (MDCK-dEREG)
CVCL_D5I0 (MDCK-dHBEGF)CVCL_D5I1 (MDCK-dTGFalpha)CVCL_AQ31 (MDCK-E [Korea])
CVCL_Y089 (MDCK-E [Taiwan])CVCL_D5IG (MDCK-EKAREV-NLS)CVCL_D5IR (MDCK-EKARrEV-NLS)
CVCL_AP75 (MDCK-F)CVCL_0592 (MDCK-I)CVCL_0424 (MDCK-II)
CVCL_IZ19 (MDCK-P-gp)CVCL_JX35 (MDCK-Protein Free)CVCL_D3UF (MDCK-RE7)
CVCL_IQ72 (MDCK-S)CVCL_IQ73 (MDCK-SF101)CVCL_IQ74 (MDCK-SF102)
CVCL_IQ75 (MDCK-SF103)CVCL_WL74 (MDCK-SFS)CVCL_Z936 (MDCK-SIAT1)
CVCL_A6IW (MDCK-UT-A1)CVCL_A2JE (MDCK-W73)CVCL_A9IZ (MDCK-XF02)
CVCL_A9JA (MDCK-XF03)CVCL_A9JB (MDCK-XF04)CVCL_B033 (MDCK.1)
CVCL_B034 (MDCK.2)CVCL_DE56 (MDCK.5F1)CVCL_2586 (MDCK.P3)
CVCL_YZ44 (MDCK.STAT1 KO)CVCL_WL70 (MDCK.SUS1)CVCL_K241 (MDCK/60)
CVCL_WC98 (MDCK/IgR)CVCL_WL76 (ssf-MDCK)CVCL_6452 (Super Dome)
CVCL_6453 (Super Tube)CVCL_E0YT (Ubigene MDCK ANP32A KO)CVCL_E0YU (Ubigene MDCK CADM1 KO)
CVCL_E0YV (Ubigene MDCK GSR KO)CVCL_E0YW (Ubigene MDCK JAM2 KO)CVCL_E0YX (Ubigene MDCK JAM3 KO)
CVCL_E0YY (Ubigene MDCK L1CAM KO)CVCL_E0YZ (Ubigene MDCK MAS1 KO)CVCL_E0Z0 (Ubigene MDCK NPHP1 KO)
CVCL_E0Z1 (Ubigene MDCK STAT1 KO)CVCL_E0Z2 (Ubigene MDCK TGM2 KO)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/Madin-Darby_Canine_Kidney_Cells
https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-ccl-34-mdck
https://www.culturecollections.org.uk/products/cell-cultures/ecacc-cell-line-profiles/mdck/
https://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/mdck/mdckcells.html
https://www.olympus-lifescience.com/de/microscope-resource/primer/techniques/fluorescence/gallery/cells/epithelialindex/
https://en.vircell.com/products/mdck-cell-line/
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-89.html
Publications

PubMed=13901412; DOI=10.1126/science.138.3536.42
Green I.J.
Serial propagation of influenza B (Lee) virus in a transmissible line of canine kidney cells.
Science 138:42-43(1962)

PubMed=5918973; DOI=10.3181/00379727-122-31293
Gaush C.R., Hard W.L., Smith T.F.
Characterization of an established line of canine kidney cells (MDCK).
Proc. Soc. Exp. Biol. Med. 122:931-935(1966)

DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=222773; DOI=10.1083/jcb.81.3.635; PMCID=PMC2110404
Rindler M.J., Chuman L.M., Shaffer L., Saier M.H. Jr.
Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK).
J. Cell Biol. 81:635-648(1979)

PubMed=6480288
Schroyens W., Bruyneel R., Tchao R., Leighton J., Dragonetti C.H., Mareel M.M.
Comparison of invasiveness and non-invasiveness of two epithelial cell lines in vitro.
Invasion Metastasis 4:160-170(1984)

PubMed=12667817; DOI=10.1016/S0042-6822(02)00064-8
Romanova J., Katinger D., Ferko B., Voglauer R., Mochalova L., Bovin N., Lim W., Katinger H., Egorov A.
Distinct host range of influenza H3N2 virus isolates in Vero and MDCK cells is determined by cell specific glycosylation pattern.
Virology 307:90-97(2003)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=21819694; PMCID=PMC3123757
Omeir R.L., Teferedegne B., Foseh G.S., Beren J.J., Snoy P.J., Brinster L.R., Cook J.L., Peden K., Lewis A.M. Jr.
Heterogeneity of the tumorigenic phenotype expressed by Madin-Darby canine kidney cells.
Comp. Med. 61:243-250(2011)

PubMed=21982418; DOI=10.1186/1471-2121-12-43; PMCID=PMC3209442
Dukes J.D., Whitley P., Chalmers A.D.
The MDCK variety pack: choosing the right strain.
BMC Cell Biol. 12:43.1-43.4(2011)

PubMed=24058646; DOI=10.1371/journal.pone.0075014; PMCID=PMC3772841
Lugovtsev V.Y., Melnyk D., Weir J.P.
Heterogeneity of the MDCK cell line and its applicability for influenza virus research.
PLoS ONE 8:E75014-E75014(2013)

PubMed=25903999; DOI=10.1002/biot.201400388
Genzel Y.
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)

PubMed=32854295; DOI=10.3390/ijms21176111; PMCID=PMC7504089
Rodrigues A.F., Fernandes P., Laske T., Castro R., Alves P.M., Genzel Y., Coroadinha A.S.
Cell bank origin of MDCK parental cells shapes adaptation to serum-free suspension culture and canine adenoviral vector production.
Int. J. Mol. Sci. 21:6111.1-6111.27(2020)

PubMed=33122286; DOI=10.1681/ASN.2020010009; PMCID=PMC7894662
Khundmiri S.J., Chen L.-H., Lederer E.D., Yang C.-R., Knepper M.A.
Transcriptomes of major proximal tubule cell culture models.
J. Am. Soc. Nephrol. 32:86-97(2021)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line collections (Providers) AddexBio; P0014003/4928
ATCC; CCL-34
ATCC; CRL-6253 - Discontinued
BCRC; 60004
BCRJ; 0168
BEI_Resources; NR-2628
CCLV; CCLV-RIE 0083
CCTCC; GDC0012
CCTCC; GDC0401
CLS; 602280
ECACC; 84121903
ECACC; 85011435
IBRC; C10556
IZSLER; BS CL 64
JCRB; IFO50071
JCRB; JCRB9029
KCB; KCB 2006105YJ
KCLB; 10034
NCBI_Iran; C426
RCB; RCB0995
Ubigene; YC-C005
Cell line databases/resources CLO; CLO_0007646
CLO; CLO_0007647
CLO; CLO_0050861
MCCL; MCC:0000319
CLDB; cl3417
CLDB; cl3418
CLDB; cl3419
CLDB; cl3420
CLDB; cl3421
CLDB; cl3422
CLDB; cl3423
CLDB; cl3424
CLDB; cl3425
CCRID; 1101CAN-PUMC000078
CCRID; 1102CAN-NIFDC00045
CCRID; 3101CANGNO23
CCRID; 4201CAN-CCTCC00012
FCS-free; 41-14-47-1-8-9
FCS-free; 41-14-321-2-3-3
FCS-free; 41-14-427-1-4-3
FCS-free; 41-14-585-1-18-2
FCS-free; 41-14-587-1-18-2
FCS-free; 41-14-588-2-18-2
Lonza; 153
TOKU-E; 2403
TOKU-E; 3636
Anatomy/cell type resources BTO; BTO:0000837
Chemistry resources ChEMBL-Cells; CHEMBL3308370
ChEMBL-Targets; CHEMBL614068
PubChem_Cell_line; CVCL_0422
Encyclopedic resources Wikidata; Q28335011
Experimental variables resources EFO; EFO_0022746
Gene expression databases GEO; GSM4009056
GEO; GSM4009057
GEO; GSM4009058
Medical resources MeSH; D061985
Proteomic databases PRIDE; PXD014768
PRIDE; PXD024031
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number39