Cellosaurus MDCK-EKARrEV-NLS (CVCL_D5IR)
Cell line name | MDCK-EKARrEV-NLS | |||
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Synonyms | MDCK-WT-EKARrEV-NLS | |||
Accession | CVCL_D5IR | |||
Resource Identification Initiative | To cite this cell line use: MDCK-EKARrEV-NLS (RRID:CVCL_D5IR) | |||
Comments | Characteristics: Transduced with the FRET biosensor EKARrEV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from RSK1, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal (PubMed=34667080). Transfected with: UniProtKB; Q66PV6; CFP. Transfected with: UniProtKB; P42212; YFP (modified GFP at G-65, L-68, A-72, Y-203 and L-231). Derived from site: In situ; Kidney; UBERON=UBERON_0002113. Cell type: Epithelial cell of kidney; CL=CL_0002518. | |||
Species of origin | Canis lupus familiaris (Dog) (Canis familiaris)
(NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel. | |||
Hierarchy | Parent: CVCL_0422 (MDCK) Children:
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Sex of cell | Female | |||
Age at sampling | Adult | |||
Category | Spontaneously immortalized cell line | |||
Publications | PubMed=34667080; DOI=10.26508/lsa.202101206 | |||
Cross-references | ||||
Cell line collections (Providers) | JCRB; JCRB1973 | |||
Entry history | ||||
Entry creation | 02-May-2024 | |||
Last entry update | 02-May-2024 | |||
Version number | 1 |