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Cellosaurus MDCK siat7e-expressing clone 2 (CVCL_D3W4)

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Cell line name MDCK siat7e-expressing clone 2
Accession CVCL_D3W4
Resource Identification Initiative To cite this cell line use: MDCK siat7e-expressing clone 2 (RRID:CVCL_D3W4)
Comments Group: Vaccine production cell line.
Characteristics: Grows in suspension (PubMed=19706449).
Virology: Allows efficient influenza virus growth and hemagglutinin (HA) production (PubMed=19706449).
Genetic integration: Method=Transfection; Gene=HGNC; 19342; ST6GALNAC5.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
Hierarchy Parent: CVCL_0422 (MDCK)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=19706449; DOI=10.1073/pnas.0905912106; PMCID=PMC2728112
Chu C., Lugovtsev V.Y., Golding H., Betenbaugh M.J., Shiloach J.
Conversion of MDCK cell line to suspension culture by transfecting with human siat7e gene and its application for influenza virus production.
Proc. Natl. Acad. Sci. U.S.A. 106:14802-14807(2009)

Cross-references
Encyclopedic resources Wikidata; Q127382755
Entry history
Entry creation30-Jan-2024
Last entry update10-Sep-2024
Version number2