Cellosaurus MDCK Actinin-M-cpstFRET (CVCL_ZV93)
| Cell line name | MDCK Actinin-M-cpstFRET |
|---|---|
| Synonyms | MDCK M-cpstFRET |
| Accession | CVCL_ZV93 |
| Resource Identification Initiative | To cite this cell line use: MDCK Actinin-M-cpstFRET (RRID:CVCL_ZV93) |
| Comments | Characteristics: Used to report forces in actinin in living cells. Has one actinin molecule with the cpstFRET sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency. Genetic integration: Method=Transfection; Gene=HGNC; 163; ACTN1. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Derived from site: In situ; Kidney; UBERON=UBERON_0002113. Cell type: Epithelial cell of kidney; CL=CL_0002518. |
| Species of origin | Canis lupus familiaris (Dog) (Canis familiaris)
(NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372. |
| Hierarchy | Parent: CVCL_0422 (MDCK) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=25422450; DOI=10.1073/pnas.1411683111; PMCID=PMC4267376 |
| Cross-references | |
| Cell line collections (Providers) | Kerafast; EBU026 |
| Encyclopedic resources | Wikidata; Q98127198 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 10-Sep-2024 |
| Version number | 6 |