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Cellosaurus HaCaT (CVCL_0038)

[Text version]
Cell line name HaCaT
Synonyms HaCAT; HACAT; Hacat
Accession CVCL_0038
Resource Identification Initiative To cite this cell line use: HaCaT (RRID:CVCL_0038)
Comments Population: Caucasian.
Virology: Susceptible to infection by Zika virus (ZIKV) (PubMed=29468137).
Doubling time: 28 hours (PubMed=32938951).
Omics: Deep antibody staining analysis.
Omics: Deep proteome analysis.
Omics: Metabolome analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Caution: Although HaCaT is distributed by a number of sources, the only official DKFZ-approved supplier is CLS.
Derived from site: In situ; Back, skin, epidermis; UBERON=UBERON_0001068+UBERON_0001003.
Cell type: Keratinocyte; CL=CL_0000312.
Sequence variations
  • Mutation; HGNC; 11998; TP53; Simple; p.His179Tyr (c.535C>T); ClinVar=VCV000127815; Zygosity=Heterozygous (PubMed=8504475).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg282Trp (c.843_844CC>TT); Zygosity=Heterozygous (PubMed=8504475).
HLA typing Source: CLS=300493
Class I
HLA-AA*31:01:02
HLA-BB*40:01:02,51:01:01
HLA-CC*03:04:01,15:02:01
Class II
HLA-DPDPB1*03:01:01,04:01:01
HLA-DQDQA1*01:02:01,03:03:01
DQB1*03:01:01,06:02:01
HLA-DRDRB1*04:01:01,15:01:01
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_XK16 (HaCaT A-5)CVCL_HD65 (HaCaT BHLHE40 (-/-))CVCL_FA29 (HaCaT HSP70B'-GFP reporter)
CVCL_3868 (HaCaT II-4)CVCL_S024 (HaCaT SimpleCell O-GalNAc)CVCL_QX09 (HaCaT siYAP)
CVCL_QX10 (HaCaT YAP-5SA)CVCL_B0AY (HaCaT-16E5)CVCL_B0AZ (HaCaT-16E6)
CVCL_B0B0 (HaCaT-16E6E7)CVCL_B0B1 (HaCaT-16E7)CVCL_B0B2 (HaCaT-18E5)
CVCL_B0B3 (HaCaT-18E6)CVCL_B0B4 (HaCaT-18E7)CVCL_B0B5 (HaCaT-62E5)
CVCL_B0B6 (HaCaT-62E6)CVCL_B0B7 (HaCaT-62E7)CVCL_B0B8 (HaCaT-84E5)
CVCL_B0B9 (HaCaT-84E6)CVCL_B0BA (HaCaT-84E7)CVCL_B6YN (HaCaT-hGSTP1 ARE-SV40p-Luc2)
CVCL_B0BB (HaCaT-pLVX)CVCL_ZW69 (HaCaT-shNeg)CVCL_ZW70 (HaCaT-shNrf2#1)
CVCL_ZW71 (HaCaT-shNrf2#2)CVCL_ZW72 (HaCaT-shNrf2#3)CVCL_RL61 (HaCaT-TRS4)
CVCL_4Z60 (HaCaT/SM)CVCL_XK18 (HaCaT40degree)CVCL_E1JZ (HyCyte HaCaT KO-hPI3)
CVCL_BW57 (NOK-SI)CVCL_A9W9 (nr-HaCat)CVCL_D9WZ (Ubigene HaCaT GJB2 KO)
Sex of cell Male
Age at sampling 62Y
Category Spontaneously immortalized cell line
STR profile Source(s): CCRID; CLS=300493; DSMZ=ACC-771; PubMed=11416159; PubMed=21868764; PubMed=35072517; Technion Genomics Center

Markers:
AmelogeninX
CSF1PO9,11
D1S165611,12
D2S44111
D2S133817,25
D3S135816
D5S81812
D6S104312,19
D7S8209,11
D8S117914
D10S124814,15
D12S39118,23
D13S31710,12
D16S5399,12
D18S5112
D19S43313,14
D21S1128 (DSMZ=ACC-771)
28,30.2 (CLS=300493; PubMed=11416159; PubMed=35072517; Technion Genomics Center)
30.2 (CCRID)
D22S104515,16
FGA24 (CCRID; DSMZ=ACC-771; PubMed=11416159; PubMed=35072517; Technion Genomics Center)
24,27.2 (CLS=300493)
Penta D11,13
Penta E7,12
SE3315,18.2
TH019.3
TPOX11,12
vWA16,17

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/HaCaT
https://www.proteinatlas.org/learn/cellines
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-347.html
Publications

PubMed=2450098; DOI=10.1083/jcb.106.3.761; PMCID=PMC2115116
Boukamp P., Dzarlieva-Petrusevska R.T., Breitkreutz D., Hornung J., Markham A.F., Fusenig N.E.
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
J. Cell Biol. 106:761-771(1988)

PubMed=8162337; DOI=10.1111/j.1600-0625.1993.tb00030.x
Schurer N., Kohne A., Schliep V., Barlag K., Goerz G.
Lipid composition and synthesis of HaCaT cells, an immortalized human keratinocyte line, in comparison with normal human adult keratinocytes.
Exp. Dermatol. 2:179-185(1993)

PubMed=8504475; DOI=10.1093/carcin/14.5.833
Lehman T.A., Modali R.V., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.
p53 mutations in human immortalized epithelial cell lines.
Carcinogenesis 14:833-839(1993)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9406811; DOI=10.1111/1523-1747.ep12340708
Henseleit U., Zhang J., Wanner R., Haase I., Kolde G., Rosenbach T.
Role of p53 in UVB-induced apoptosis in human HaCaT keratinocytes.
J. Invest. Dermatol. 109:722-727(1997)

PubMed=10350023; DOI=10.1023/A:1011945212831
Altenburger R., Kissel T.
The human keratinocyte cell line HaCaT: an in vitro cell culture model for keratinocyte testosterone metabolism.
Pharm. Res. 16:766-771(1999)

PubMed=10523843; DOI=10.1038/sj.onc.1202934
Boukamp P., Popp S., Bleuel K., Tomakidi E., Burkle A., Fusenig N.E.
Tumorigenic conversion of immortal human skin keratinocytes (HaCaT) by elevated temperature.
Oncogene 18:5638-5645(1999)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=11583982; DOI=10.1016/S0002-9440(10)62541-2; PMCID=PMC1850484
Mueller M.M., Peter W., Mappes M., Huelsen A., Steinbauer H., Boukamp P., Vaccariello M.A., Garlick J.A., Fusenig N.E.
Tumor progression of skin carcinoma cells in vivo promoted by clonal selection, mutagenesis, and autocrine growth regulation by granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor.
Am. J. Pathol. 159:1567-1579(2001)

PubMed=15389883; DOI=10.1002/jcb.20212
Lemaitre G., Lamartine J., Pitaval A., Vaigot P., Garin J., Bouet S., Petat C., Soularue P., Gidrol X., Martin M.T., Waksman G.
Expression profiling of genes and proteins in HaCaT keratinocytes: proliferating versus differentiated state.
J. Cell. Biochem. 93:1048-1062(2004)

PubMed=20097197; DOI=10.1016/j.yexcr.2010.01.020
Rasanen K., Vaheri A.
Proliferation and motility of HaCaT keratinocyte derivatives is enhanced by fibroblast nemosis.
Exp. Cell Res. 316:1739-1747(2010)

PubMed=20100199; DOI=10.1111/j.1600-0625.2009.01026.x; PMCID=PMC2891305
Olaru F., Jensen L.E.
Chemokine expression by human keratinocyte cell lines after activation of Toll-like receptors.
Exp. Dermatol. 19:e314-e316(2010)

PubMed=21185708; DOI=10.1016/j.jnutbio.2010.08.004
Serini S., Donato V., Piccioni E., Trombino S., Monego G., Toesca A., Innocenti I., Missori M., De Spirito M., Celleno L., Fasano E., Ranelletti F.O., Calviello G.
Docosahexaenoic acid reverts resistance to UV-induced apoptosis in human keratinocytes: involvement of COX-2 and HuR.
J. Nutr. Biochem. 22:874-885(2011)

PubMed=21269460; DOI=10.1186/1752-0509-5-17; PMCID=PMC3039570
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.
Initial characterization of the human central proteome.
BMC Syst. Biol. 5:17.1-17.13(2011)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690; PMCID=PMC3229662
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.
Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.
Clin. Cancer Res. 17:7248-7264(2011)

PubMed=24155234; DOI=10.1007/7651_2013_42
Wilson V.G.
Growth and differentiation of HaCaT keratinocytes.
Methods Mol. Biol. 1195:33-41(2014)

PubMed=26989177; DOI=10.1242/dev.133728; PMCID=PMC4874484
Elbediwy A., Vincent-Mistiaen Z.I., Spencer-Dene B., Stone R.K., Boeing S., Wculek S.K., Cordero J., Tan E.H., Ridgway R., Brunton V.G., Sahai E., Gerhardt H., Behrens A., Malanchi I., Sansom O.J., Thompson B.J.
Integrin signalling regulates YAP and TAZ to control skin homeostasis.
Development 143:1674-1687(2016)

PubMed=29468137; DOI=10.5501/wjv.v7.i1.10; PMCID=PMC5807893
Himmelsbach K., Hildt E.
Identification of various cell culture models for the study of Zika virus.
World J. Virol. 7:10-20(2018)

PubMed=29660373; DOI=10.1016/j.bbagen.2018.04.012
Touat-Hamici Z., Bulteau A.-L., Bianga J., Jean-Jacques H., Szpunar J., Lobinski R., Chavatte L.
Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines.
Biochim. Biophys. Acta 1862:2493-2505(2018)

PubMed=31629070; DOI=10.1016/j.tiv.2019.104666
da Silva A.C.G., Chialchia A.R., de Castro E.G., e Silva M.R.L., Arantes D.A.C., Batista A.C., Kitten G.T., Valadares M.C.
A new corneal epithelial biomimetic 3D model for in vitro eye toxicity assessment: development, characterization and applicability.
Toxicol. In Vitro 62:104666.1-104666.7(2020)

PubMed=32938951; DOI=10.1038/s41598-020-71315-0; PMCID=PMC7494900
Pavez Lorie E., Stricker N., Plitta-Michalak B.P., Chen I.-P., Volkmer B., Greinert R., Jauch A., Boukamp P., Rapp A.
Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw.
Sci. Rep. 10:15196-15196(2020)

PubMed=33718544; DOI=10.1016/j.dib.2021.106871; PMCID=PMC7921465
Rusanov A.L., Romashin D.D., Zgoda V.G., Butkova T.V., Luzgina N.G.
Protein dataset of immortalized keratinocyte HaCaT cells and normal human keratinocytes.
Data Brief 35:106871.1-106871.6(2021)

PubMed=35072517; DOI=10.1289/EHP9676; PMCID=PMC8785870
Ferragut Cardoso A.P., Banerjee M., Al-Eryani L., Sayed M., Wilkey D.W., Merchant M.L., Park J.W., States J.C.
Temporal modulation of differential alternative splicing in HaCaT human keratinocyte cell line chronically exposed to arsenic for up to 28 wk.
Environ. Health Perspect. 130:17011.1-17011.16(2022)

PubMed=35647242; DOI=10.1016/j.dib.2022.108274; PMCID=PMC9130082
Romashin D.D., Rusanov A.L., Kozhin P.M., Karagyaur M.N., Tikhonova O.V., Zgoda V.G., Luzgina N.G.
Impact of p53 knockdown on protein dataset of HaCaT cells.
Data Brief 42:108274.1-108274.8(2022)

Cross-references
Cell line collections (Providers) AddexBio; T0020001/117
BCRJ; 0341 - Discontinued
CCTCC; GDC0106
CLS; 300493
DSMZ; ACC-771 - Discontinued
ICLC; HL14001 - Discontinued
IZSLER; BS CL 168
KCB; KCB 200442YJ
Ubigene; YC-A018
Cell line databases/resources CLDB; cl7229
CCRID; 1101HUM-PUMC000373
CCRID; 4201HUM-CCTCC00106
DSMZCellDive; ACC-771
Lonza; 56
TOKU-E; 3890
Anatomy/cell type resources BTO; BTO:0000552
Chemistry resources ChEMBL-Cells; CHEMBL3308509
ChEMBL-Targets; CHEMBL614392
PubChem_Cell_line; CVCL_0038
Encyclopedic resources Wikidata; Q5636366
Experimental variables resources EFO; EFO_0002056
Gene expression databases GEO; GSE57080
GEO; GSM913344
GEO; GSM913345
GEO; GSM960278
GEO; GSM960279
GEO; GSM960280
GEO; GSM960286
GEO; GSM960287
GEO; GSM960288
GEO; GSM960294
GEO; GSM960295
GEO; GSM960296
GEO; GSM1375594
GEO; GSM1375595
GEO; GSM1375596
GEO; GSM1375597
GEO; GSM1375598
GEO; GSM1375599
GEO; GSM1375600
GEO; GSM1375601
GEO; GSM1375602
GEO; GSM1375603
GEO; GSM1375604
GEO; GSM1375605
GEO; GSM1861816
GEO; GSM1861817
GEO; GSM1861820
GEO; GSM1861821
GEO; GSM2112559
GEO; GSM2112563
GEO; GSM2495796
GEO; GSM2495797
GEO; GSM2495798
Medical resources MeSH; D000084282
Metabolomic databases MetaboLights; MTBLS127
Polymorphism and mutation databases Cosmic; 1530737
IARC_TP53; 1820
Proteomic databases PRIDE; PXD000496
PRIDE; PXD000670
PRIDE; PXD002460
PRIDE; PXD007789
PRIDE; PXD013113
PRIDE; PXD017536
PRIDE; PXD033538
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number42