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Cellosaurus HaCaT HSP70B'-GFP reporter (CVCL_FA29)

[Text version]
Cell line name HaCaT HSP70B'-GFP reporter
Accession CVCL_FA29
Resource Identification Initiative To cite this cell line use: HaCaT HSP70B'-GFP reporter (RRID:CVCL_FA29)
Comments Population: Caucasian.
Characteristics: Stably expresses GFP under the control of the stress-inducible HSPA6 (HSP70B') promoter.
Characteristics: Used as a sensor cell line to quantify skin response upon exposure to chemical substances that are known to cause irritation or allergies through the generation of reactive oxygen species.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Derived from site: In situ; Back, skin, epidermis; UBERON=UBERON_0001068+UBERON_0001003.
Cell type: Keratinocyte; CL=CL_0000312.
Sequence variations
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.His179Tyr (c.535C>T); ClinVar=VCV000127815; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg282Trp (c.843_844CC>TT); Zygosity=Heterozygous (from parent cell line).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0038 (HaCaT)
Sex of cell Male
Age at sampling 62Y
Category Spontaneously immortalized cell line
Publications

PubMed=24967604; DOI=10.3390/s140711293; PMCID=PMC4168474
Hofmann U., Priem M., Bartzsch C., Winckler T., Feller K.-H.
A sensitive sensor cell line for the detection of oxidative stress responses in cultured human keratinocytes.
Sensors (Basel) 14:11293-11307(2014)

Cross-references
Encyclopedic resources Wikidata; Q54872311
Entry history
Entry creation26-Sep-2016
Last entry update19-Dec-2024
Version number15