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Cellosaurus HaCaT A-5 (CVCL_XK16)

[Text version]
Cell line name HaCaT A-5
Synonyms HaCaT-ras clone A-5; HaCaT-ras A5; A-5; A5
Accession CVCL_XK16
Resource Identification Initiative To cite this cell line use: HaCaT A-5 (RRID:CVCL_XK16)
Comments Population: Caucasian.
Characteristics: Non-tumorigenic.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:5173; HRAS.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Back, skin, epidermis; UBERON=UBERON_0001068+UBERON_0001003.
Cell type: Keratinocyte; CL=CL_0000312.
Sequence variations
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.His179Tyr (c.535C>T); ClinVar=VCV000127815; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg282Trp (c.843_844CC>TT); Zygosity=Heterozygous (from parent cell line).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0038 (HaCaT)
Children:
CVCL_XK15 (HaCaT RT3)
Sex of cell Male
Age at sampling 62Y
Category Spontaneously immortalized cell line
STR profile Source(s): CLS=300494

Markers:
AmelogeninX
CSF1PO9,11
D1S165611,12
D2S44111
D2S133817,25
D3S135816
D5S81812
D7S8209,11
D8S117914
D10S124814,15
D12S39118,23
D13S31710,12
D16S5399,12
D18S5112
D19S43313,14
D21S1128,30.2
D22S104515,16
FGA24,27.2
SE3315,18.2
TH019.3
TPOX11,12
vWA16,17

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Publications

PubMed=11583982; DOI=10.1016/S0002-9440(10)62541-2; PMCID=PMC1850484
Mueller M.M., Peter W., Mappes M., Huelsen A., Steinbauer H., Boukamp P., Vaccariello M.A., Garlick J.A., Fusenig N.E.
Tumor progression of skin carcinoma cells in vivo promoted by clonal selection, mutagenesis, and autocrine growth regulation by granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor.
Am. J. Pathol. 159:1567-1579(2001)

PubMed=20097197; DOI=10.1016/j.yexcr.2010.01.020
Rasanen K., Vaheri A.
Proliferation and motility of HaCaT keratinocyte derivatives is enhanced by fibroblast nemosis.
Exp. Cell Res. 316:1739-1747(2010)

Cross-references
Cell line collections (Providers) CLS; 300494
Encyclopedic resources Wikidata; Q93937678
Proteomic databases PRIDE; PXD013113
Entry history
Entry creation19-Dec-2019
Last entry update19-Dec-2024
Version number11