Cellosaurus cell line NOK-SI (CVCL

Cellosaurus NOK-SI (CVCL_BW57)

[Text version]

Cell line name

NOK-SI

Synonyms

NOKSI; NOKsi; Normal Oral Keratinocytes-Spontaneously Immortalized

Accession

CVCL_BW57

Resource Identification Initiative

To cite this cell line use: NOK-SI (RRID:CVCL_BW57)

Comments

Problematic cell line: Contaminated. Shown to be a HaCaT derivative (ICLAC). Originally thought to originate from normal oral keratinocytes from a female donor.
Part of: OPC-22 oral and pharyngeal cancer cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00589.
Population: Caucasian.
Omics: Deep exome analysis.
Derived from site: In situ; Back, skin, epidermis; UBERON=UBERON_0001068+UBERON_0001003.
Cell type: Keratinocyte; CL=CL_0000312.

Sequence variations

Mutation; HGNC; 11998; TP53; Simple; p.His179Tyr (c.535C>T); ClinVar=VCV000127815; Zygosity=Unspecified (PubMed=25275298).

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0038 (HaCaT)

Sex of cell

Male

Age at sampling

62Y

Category

Spontaneously immortalized cell line

STR profile

Source(s): PubMed=25275298

Markers:

Amelogenin	X
CSF1PO	9,11
D3S1358	16
D5S818	12
D7S820	9,11
D8S1179	14
D13S317	10,12
D16S539	9,12
D18S51	12
D21S11	28,30.2
FGA	24
Penta D	11,13
Penta E	7,12
TH01	9.3
TPOX	11,12
vWA	16,17

Web pages

https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx

Publications

PubMed=20463891; DOI=10.1371/journal.pone.0010503; PMCID=PMC2865533
Castilho R.M., Squarize C.H., Leelahavanichkul K., Zheng Y., Bugge T., Gutkind J.S.
Rac1 is required for epithelial stem cell function during dermal and oral mucosal wound healing but not for tissue homeostasis in mice.
PLoS ONE 5:E10503-E10503(2010)

PubMed=25275298; DOI=10.18632/oncotarget.2417; PMCID=PMC4253406
Martin D., Abba M.C., Molinolo A.A., Vitale-Cross L., Wang Z.-Y., Zaida M., Delic N.C., Samuels Y., Lyons J.G., Gutkind J.S.
The head and neck cancer cell oncogenome: a platform for the development of precision molecular therapies.
Oncotarget 5:8906-8923(2014)

Cross-references

Encyclopedic resources

Wikidata; Q54930891

Polymorphism and mutation databases

Cosmic; 2296312

Entry history

Entry creation

02-May-2016

Last entry update

02-May-2024

Version number