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Cellosaurus 46C (CVCL_Y482)

[Text version]
Cell line name 46C
Synonyms ES-46C; ES46C; 46C ES
Accession CVCL_Y482
Resource Identification Initiative To cite this cell line use: 46C (RRID:CVCL_Y482)
Comments Part of: ENCODE project mouse cell lines.
Characteristics: Using homologous recombination the Sox1 reading frame was replaced by a GFP-IRES-PuroR construct. Expresses eGFP under the control of the Sox-1 promoter (PubMed=12524553).
Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: 129P2/Ola.
Hierarchy Parent: CVCL_Y481 (ES-E14TG2a.4)
Children:
CVCL_ZE88 (46C-Dip2a KO)CVCL_A7IR (46C-Dip2c KO)CVCL_YJ62 (46C-Rybp KO)
CVCL_D1HI (NS-5)CVCL_A5EK (NS46C)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Web pages https://biokryo.airliquide.com/sites/biokryo/files/2022-07/animal-stem-cells-catalogue-isenet.pdf
Publications

PubMed=12524553; DOI=10.1038/nbt780
Ying Q.-L., Stavridis M., Griffiths D., Li M., Smith A.G.
Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture.
Nat. Biotechnol. 21:183-186(2003)

PubMed=14696356; DOI=10.1016/S0076-6879(03)65023-8
Ying Q.-L., Smith A.G.
Defined conditions for neural commitment and differentiation.
Methods Enzymol. 365:327-341(2003)

PubMed=21454627; DOI=10.1074/jbc.M110.210740; PMCID=PMC3099688
Cardano M., Diaferia G.R., Cattaneo M., Dessi S.S., Long Q.-M., Conti L., DeBlasio P., Cattaneo E., Biunno I.
mSEL-1L (suppressor/enhancer Lin12-like) protein levels influence murine neural stem cell self-renewal and lineage commitment.
J. Biol. Chem. 286:18708-18719(2011)

Cross-references
Biological sample resources ENCODE; ENCBS122ENC
Encyclopedic resources Wikidata; Q54603238
Experimental variables resources EFO; EFO_0005484
Gene expression databases GEO; GSM450272
GEO; GSM450283
Entry history
Entry creation07-Nov-2014
Last entry update19-Dec-2024
Version number14