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Cellosaurus 46C-Dip2c KO (CVCL_A7IR)

[Text version]
Cell line name 46C-Dip2c KO
Synonyms IBMSe001-B-1
Accession CVCL_A7IR
Resource Identification Initiative To cite this cell line use: 46C-Dip2c KO (RRID:CVCL_A7IR)
Comments Characteristics: Using CRISPR/Cas9 exon 8 of Dip2c was replaced by a PGKp-PuroR-P2A-mCherry construct (PubMed=33813174).
Characteristics: Using homologous recombination the Sox1 reading frame was replaced by a GFP-IRES-PuroR construct. Expresses eGFP under the control of the Sox-1 promoter (from parent cell line).
Knockout cell: Method=CRISPR/Cas9; MGI; MGI:1920179; Dip2c.
Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: 129P2/Ola.
Hierarchy Parent: CVCL_Y482 (46C)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=33813174; DOI=10.1016/j.scr.2021.102236
Yao M.-Z., Su P.-F., Li Z.-F., Cui X.-G., Yang Q., Xing X.-Y., Lei Y., Lian M.-Q., Ren T.-L., Wang X.-D., Zheng Y.-W., Wu C.-X.
Knockout of Dip2c in murine ES cell line IBMSe001-B-1 by CRISPR/Cas9 genome editing technology.
Stem Cell Res. 53:102236-102236(2021)

Cross-references
Encyclopedic resources Wikidata; Q107113284
Entry history
Entry creation20-May-2021
Last entry update19-Dec-2024
Version number6