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Cellosaurus NS46C (CVCL_A5EK)

[Text version]
Cell line name NS46C
Synonyms LC-1; LC1
Accession CVCL_A5EK
Resource Identification Initiative To cite this cell line use: NS46C (RRID:CVCL_A5EK)
Comments Characteristics: Using homologous recombination the Sox1 reading frame was replaced by a GFP-IRES-PuroR construct. Expresses eGFP under the control of the Sox-1 promoter (from parent cell line).
Doubling time: ~24 hours (PubMed=16086633).
Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Neural stem cell; CL=CL_0000047.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: 129P2/Ola.
Hierarchy Parent: CVCL_Y482 (46C)
Sex of cell Male
Age at sampling Blastocyst stage
Category Somatic stem cell
Web pages https://biokryo.airliquide.com/sites/biokryo/files/2022-07/animal-stem-cells-catalogue-isenet.pdf
Publications

PubMed=16086633; DOI=10.1371/journal.pbio.0030283; PMCID=PMC1184591
Conti L., Pollard S.M., Gorba T., Reitano E., Toselli M., Biella G., Sun Y.-R., Sanzone S., Ying Q.-L., Cattaneo E., Smith A.G.
Niche-independent symmetrical self-renewal of a mammalian tissue stem cell.
PLoS Biol. 3:e283.1594-e283.1606(2005)

PubMed=21454627; DOI=10.1074/jbc.M110.210740; PMCID=PMC3099688
Cardano M., Diaferia G.R., Cattaneo M., Dessi S.S., Long Q.-M., Conti L., DeBlasio P., Cattaneo E., Biunno I.
mSEL-1L (suppressor/enhancer Lin12-like) protein levels influence murine neural stem cell self-renewal and lineage commitment.
J. Biol. Chem. 286:18708-18719(2011)

Cross-references
Encyclopedic resources Wikidata; Q107116581
Entry history
Entry creation20-May-2021
Last entry update19-Dec-2024
Version number8