Cellosaurus 46C-Dip2a KO (CVCL_ZE88)
| Cell line name | 46C-Dip2a KO |
|---|---|
| Synonyms | IBMSe001-A-1 |
| Accession | CVCL_ZE88 |
| Resource Identification Initiative | To cite this cell line use: 46C-Dip2a KO (RRID:CVCL_ZE88) |
| Comments | Characteristics: Using CRISPR/Cas9 exon 8 of Dip2a was replaced by a PGKp-PuroR-P2A-mCherry construct (PubMed=32361465). Characteristics: Using homologous recombination the Sox1 reading frame was replaced by a GFP-IRES-PuroR construct. Expresses eGFP under the control of the Sox-1 promoter (from parent cell line). Knockout cell: Method=CRISPR/Cas9; MGI; MGI:2385920; Dip2a. Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: 129P2/Ola. |
| Hierarchy | Parent: CVCL_Y482 (46C) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=32361465; DOI=10.1016/j.scr.2020.101778 |
| Cross-references | |
| Cell line databases/resources | SKIP; SKIP005729 |
| Encyclopedic resources | Wikidata; Q98125404 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 19-Dec-2024 |
| Version number | 8 |