Molecular Cytogenetics of Common Epithelial Cancers > Colon Carcinoma cell lines
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The following colon cancer cell lines have been analysed by SKY [1, 2], with in most cases verification of the SKY by 2- and 3-colour conventional FISH. The linked pages show representative SKY karyotypes and some additional comment.
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Microsatellite-stable
lines |
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Microsatellite-unstable
lines |
ref |
Microsatellite
status unknown |
ref |
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1 |
1,2,4,5 |
2,3 |
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1 |
1 |
2,3 |
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1 |
1 |
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1 |
1,
4 |
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1 |
1 |
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1 |
1 |
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1 |
1 |
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1 |
1 |
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1 |
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CGH of the 17 lines is reported in [1]
References and comments
[1] Abdel-Rahman WM, Katsura K, Rens W, Gorman PA, Sheer D, Bicknell D, Bodmer WF, Arends MJ, Wyllie AH, Edwards PAW. Spectral karyotyping suggests additional subsets of colorectal cancers characterized by pattern of chromosome rearrangement. Proc Natl Acad Sci U S A. 2001;98(5):2538-2543. PMID: 11226274
[2] Grigorova M, Staines JM, Ozdag H, Caldas C, Edwards PAW. Possible causes of chromosome instability: comparison of chromosomal abnormalities in cancer cell lines with mutations in BRCA1, BRCA2, CHK2 and BUB1. Cytogenet. Genome Res. 2004;104(1-4):333-340. PMID: 15162061
[3] VACO400 and 429 have mutations in Bub1; DLD-1 and HCT-15 are from the same tumour and have biallelic inactivation of CHK2.
[4] Chen TR, Dorotinsky CS, McGuire LJ, Macy ML, Hay RJ. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin. Cancer Genet. Cytogenet. 1995;81(2):103-108. PMID: 7621404
[5] Ghadimi BM, Sackett DL, Difilippantonio MJ, Schrock E, Neumann T, Jauho A, Auer G, Ried T. Centrosome amplification and instability occurs exclusively in aneuploid, but not in diploid colorectal cancer cell lines, and correlates with numerical chromosomal aberrations. Genes Chromosomes Cancer 2000;27:183-190. PMID: 10612807