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Cellosaurus HEp-2 (CVCL_1906)

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Cell line name HEp-2
Synonyms Hep-2; HEP-2; Hep2 (HeLa derivative); HEp-2/HeLa; Hep 2; Hep2; HEp2; HEP2; H.Ep.-2; H.Ep. #2; H.Ep. No. 2; Hep II; Human Epidermoid carcinoma #2; Human Epithelioma-2
Accession CVCL_1906
Resource Identification Initiative To cite this cell line use: HEp-2 (RRID:CVCL_1906)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=566722; PubMed=1246601; PubMed=20143388). Originally thought to originate from a 56 year old male patient with a laryngeal carcinoma.
Part of: KuDOS 95 cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00007.
Population: African American.
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Genome sequenced (low read coverage).
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Omics: Ubiquitination analysis by proteomics.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
HLA typing Source: PubMed=26589293
Class I
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
CVCL_WH94 (Ban)CVCL_W888 (DRHEp2)CVCL_2817 (HEp-2 clone 2B)
CVCL_2940 (HEp-2C)CVCL_RX04 (HEp2-Mx2/EGFP L1D7)CVCL_RX05 (HEp2-Mx2/EGFP L1G5)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CCRID; CLS; KCLB; PubMed=11416159; PubMed=17254797; RCB; TKG

D13S31712 (RCB)
12,13.3 (AddexBio; ATCC; CCRID; CLS; KCLB; TKG)
12,14 (PubMed=17254797)
D18S5115 (PubMed=17254797)
16 (CCRID; CLS; PubMed=11416159)
D21S1126,27 (PubMed=17254797)
27,28 (CCRID; CLS; PubMed=11416159)
Penta D8,15
Penta E7,17

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-204.html

Toolan H.W.
Transplantable human neoplasms maintained in cortisone-treated laboratory animals: H.S. No. 1; H.Ep. No. 1; H.Ep. No. 2; H.Ep. No. 3 and H.Emb.Rh. No. 1.
Cancer Res. 14:660-666(1954)

Moore A.E., Sabachewsky L., Toolan H.W.
Culture characteristics of four permanent lines of human cancer cells.
Cancer Res. 15:598-602(1955)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

Gartler S.M.
Genetic markers as tracers in cell culture.
Natl. Cancer Inst. Monogr. 26:167-195(1967)

PubMed=5641128; DOI=10.1038/217750a0
Gartler S.M.
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

PubMed=5668122; DOI=10.3181/00379727-128-33119
Peterson W.D. Jr., Stulberg C.S., Swanborg N.K., Robinson A.R.
Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms.
Proc. Soc. Exp. Biol. Med. 128:772-776(1968)

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

Petricciani J.C., Wallace R.E., McCoy D.W.
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=1246601; DOI=10.1126/science.1246601
Nelson-Rees W.A., Flandermeyer R.R.
HeLa cultures defined.
Science 191:96-98(1976)

PubMed=566722; DOI=10.1007/BF02616110
Lavappa K.S.
Survey of ATCC stocks of human cell lines for HeLa contamination.
In Vitro 14:469-475(1978)

PubMed=3180844; DOI=10.1159/000132579
Chen T.-R.
Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes.
Cytogenet. Cell Genet. 48:19-24(1988)

Ogura H., Yoshinouchi M., Kudo T., Imura M., Fujiwara T., Yabe Y.
Human papillomavirus type 18 DNA in so-called HEP-2, KB and FL cells -- further evidence that these cells are HeLa cell derivatives.
Cell. Mol. Biol. 39:463-467(1993)

Tsuboi I., Taguchi M., Kenmotsu M., Ban F., Machida K., Inagaki K., Ishi A., Tazawa T.
A new substrain derived from HEp-2 for the detection of anti-nuclear antibodies.
Tissue Cult. Res. Commun. 17:95-100(1998)

PubMed=17254797; DOI=10.1016/j.biologicals.2006.10.001
Azari S., Ahmadi N., Tehrani M.J., Shokri F.
Profiling and authentication of human cell lines using short tandem repeat (STR) loci: report from the National Cell Bank of Iran.
Biologicals 35:195-202(2007)

PubMed=19450234; DOI=10.2144/000113089
Rahbari R., Sheahan T., Modes V., Collier P., Macfarlane C.M., Badge R.M.
A novel L1 retrotransposon marker for HeLa cell line identification.
BioTechniques 46:277-284(2009)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=23925245; DOI=10.1038/nature12064
Adey A., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P., Martin B.K., Qiu R., Lee C., Shendure J.
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

Xu Z.-H., Sun W.-W., Hang J.-B., Gao B.-L., Hu J.-A.
RNA-seq identified a novel functional MET fusion in head and neck cancer 5-8F cell line.
Int. J. Clin. Exp. Pathol. 9:8308-8317(2016)

PubMed=29483928; DOI=10.1186/s11658-017-0067-8
Li G.-D., Hu X.-L., Sun L., Li X., Li J.-F., Li T.-L., Zhang X.-H.
C-fos upregulates P-glycoprotein, contributing to the development of multidrug resistance in HEp-2 laryngeal cancer cells with VCR-induced resistance.
Cell. Mol. Biol. Lett. 23:6.1-6.12(2018)

PubMed=29967540; DOI=10.1038/s41594-018-0084-y
Akimov V., Barrio-Hernandez I., Hansen S.V.F., Hallenborg P., Pedersen A.-K., Bekker-Jensen D.B., Puglia M., Christensen S.D.K., Vanselow J.T., Nielsen M.M., Kratchmarova I., Kelstrup C.D., Olsen J.V., Blagoev B.
UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites.
Nat. Struct. Mol. Biol. 25:631-640(2018)

Gorphe P.
A comprehensive review of Hep-2 cell line in translational research for laryngeal cancer.
Am. J. Cancer Res. 9:644-649(2019)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

PubMed=34024160; DOI=10.2144/btn-2020-0162
Fusi M., Dotti S.
Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth.
BioTechniques 70:319-326(2021)

PubMed=34697958; DOI=10.1177/24725552211051963
Korch C.T., Capes-Davis A.
The extensive and expensive impacts of HEp-2 [HeLa], intestine 407 [HeLa], and other false cell lines in journal publications.
SLAS Discov. 26:1268-1279(2021)

Cell line collections (Providers) AddexBio; C0050001/4983
BCRC; 60042 - Discontinued
BCRJ; 0101
CLS; 300397
ECACC; 86030501
KCLB; 10023
NCBI_Iran; C144
RCB; RCB1889
TKG; TKG 0403
Cell line databases/resources CLO; CLO_0003706
CLO; CLO_0003707
CLO; CLO_0050927
CLDB; cl1646
CLDB; cl1647
CLDB; cl1649
CLDB; cl1650
CLDB; cl1653
CLDB; cl1654
CLDB; cl1655
CCRID; 1101HUM-PUMC000068
Cell_Model_Passport; SIDM01208
TOKU-E; 1442
Anatomy/cell type resources BTO; BTO:0000976
Biological sample resources BioSample; SAMN03151706
Chemistry resources ChEMBL-Cells; CHEMBL3307694
ChEMBL-Targets; CHEMBL614735
PharmacoDB; HEp2_540_2019
PubChem_Cell_line; CVCL_1906
Encyclopedic resources Wikidata; Q16554737
Experimental variables resources EFO; EFO_0006438
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSM1374529
GEO; GSM1374530
Polymorphism and mutation databases Cosmic; 1339928
Cosmic; 1530739
Cosmic; 1969495
Cosmic; 2301559
Proteomic databases PRIDE; PXD006201
PRIDE; PXD013072
PRIDE; PXD014913
PRIDE; PXD031726
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number39