| Publication number |
CLPUB00484 |
|---|
| Authors |
Xu Z.-H., Sun W.-W., Hang J.-B., Gao B.-L., Hu J.-A. |
|---|
| Title |
RNA-seq identified a novel functional MET fusion in head and neck cancer 5-8F cell line. |
|---|
| Citation |
Int. J. Clin. Exp. Pathol. 9:8308-8317(2016) |
|---|
| Web pages |
https://www.ijcep.com/files/ijcep0020169.pdf |
|---|
| Abstract |
The mortality of head and neck squamous cell carcinoma (HNSCC) has
remained nearly unchanged for the past two decades. Deepening our
understanding of genomic alterations and their influence on biological
function of HNSCC cells will help develop effective and personalized
treatment. In present study, paired-end whole-transcriptome sequencing
(RNA-seq) was performed across 8 HNSCC cell lines. A novel MET fusion,
CAPZA2-MET fusion, was identified by fusion analysis with FusionMap
software and validated by nested reverse transcription-polymerase chain
reaction (RT-PCR) and Sanger sequencing in HNSCC 5-8F cell line. 5-8F
cells harboring this fusion were very sensitive to tivantinib, a specific
small molecular inhibitor to MET kinase, demonstrating that this novel MET
fusion may serve as a potential therapeutic target for HNSCC patients.
|
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| Cell lines |
CVCL_C528; 5-8F CVCL_6889; CNE-2 CVCL_1218; FaDu CVCL_1906; HEp-2 CVCL_0308; HNE-1 CVCL_FA07; HNE-2 CVCL_8706; HONE-1 CVCL_0372; KB |
|---|
