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Cellosaurus KB (CVCL_0372)

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Cell line name KB
Synonyms KB (HeLa derivative); HeLa-KB; Strain KB
Accession CVCL_0372
Resource Identification Initiative To cite this cell line use: KB (RRID:CVCL_0372)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=1246601; PubMed=20143388). Originally thought to originate from an epidermal carcinoma of the mouth.
Part of: KuDOS 95 cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00010.
Population: African American.
Doubling time: ~30 hours (DSMZ=ACC-136).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Genome sequenced (low read coverage).
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from sampling site: Uterus; cervix.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
CVCL_D680 (KB 16)CVCL_D681 (KB 18)CVCL_C4YP (KB N10)
CVCL_2088 (KB-3-1)CVCL_M096 (KB-R)CVCL_HA90 (KB/BF-1)
CVCL_D599 (KBv200)CVCL_U657 (KE2a)CVCL_6B18 (Kst-6)
CVCL_X217 (VJ-300)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; CLS; DSMZ; JCRB; KCLB; PubMed=11416159; PubMed=17254797; TKG

D13S31712,13.3 (ATCC; CLS; DSMZ; JCRB; KCLB; TKG)
12,14 (CCRID; PubMed=17254797)
Penta D8,15
Penta E7,17

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/k/cell-lines-detail-494.html

PubMed=13254761; DOI=10.3181/00379727-89-21811
Eagle H.
Propagation in a fluid medium of a human epidermoid carcinoma, strain KB.
Proc. Soc. Exp. Biol. Med. 89:362-364(1955)

PubMed=13322936; DOI=10.3181/00379727-91-22263
Eagle H., Habel K., Rowe W.P., Huebner R.J.
Viral susceptibility of a human carcinoma cell (strain KB).
Proc. Soc. Exp. Biol. Med. 91:361-364(1956)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

Gartler S.M.
Genetic markers as tracers in cell culture.
Natl. Cancer Inst. Monogr. 26:167-195(1967)

PubMed=5641128; DOI=10.1038/217750a0
Gartler S.M.
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

PubMed=5668122; DOI=10.3181/00379727-128-33119
Peterson W.D. Jr., Stulberg C.S., Swanborg N.K., Robinson A.R.
Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms.
Proc. Soc. Exp. Biol. Med. 128:772-776(1968)

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

Petricciani J.C., Wallace R.E., McCoy D.W.
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=1246601; DOI=10.1126/science.1246601
Nelson-Rees W.A., Flandermeyer R.R.
HeLa cultures defined.
Science 191:96-98(1976)

PubMed=656543; DOI=10.1016/S0006-3495(78)85487-3
Ehmann U.K., Cook K.H., Friedberg E.C.
The kinetics of thymine dimer excision in ultraviolet-irradiated human cells.
Biophys. J. 22:249-264(1978)

Ogura H., Yoshinouchi M., Kudo T., Imura M., Fujiwara T., Yabe Y.
Human papillomavirus type 18 DNA in so-called HEP-2, KB and FL cells -- further evidence that these cells are HeLa cell derivatives.
Cell. Mol. Biol. 39:463-467(1993)

PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=17254797; DOI=10.1016/j.biologicals.2006.10.001
Azari S., Ahmadi N., Tehrani M.J., Shokri F.
Profiling and authentication of human cell lines using short tandem repeat (STR) loci: report from the National Cell Bank of Iran.
Biologicals 35:195-202(2007)

PubMed=17325662; DOI=10.1038/sj.onc.1210330
Nakaya K., Yamagata H.D., Arita N., Nakashiro K.-I., Nose M., Miki T., Hamakawa H.
Identification of homozygous deletions of tumor suppressor gene FAT in oral cancer using CGH-array.
Oncogene 26:5300-5308(2007)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=23925245; DOI=10.1038/nature12064
Adey A., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P., Martin B.K., Qiu R., Lee C., Shendure J.
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)

Xu Z.-H., Sun W.-W., Hang J.-B., Gao B.-L., Hu J.-A.
RNA-seq identified a novel functional MET fusion in head and neck cancer 5-8F cell line.
Int. J. Clin. Exp. Pathol. 9:8308-8317(2016)

PubMed=28455420; DOI=10.1158/0008-5472.CAN-16-2258
Vaughan L., Glanzel W., Korch C.T., Capes-Davis A.
Widespread use of misidentified cell line KB (HeLa): incorrect attribution and its impact revealed through mining the scientific literature.
Cancer Res. 77:2784-2788(2017)

Cell line collections ATCC; CCL-17
BCRC; 60017 - Discontinued
BCRJ; 0128
CLS; 300446
DSMZ; ACC-136 - Discontinued
ECACC; 86103004 - Discontinued
ECACC; 94050408
ICLC; HTL96014 - Discontinued
KCB; KCB 94020YJ
KCLB; 10017
NCBI_Iran; C152
RCB; RCB1888 - Discontinued
TKG; TKG 0401
Cell line databases/resources CLO; CLO_0007074
MCCL; MCC:0000267
CLDB; cl3001
CLDB; cl3002
CLDB; cl3003
CLDB; cl3004
CLDB; cl3005
CLDB; cl3006
CCRID; 1101HUM-PUMC000163
DSMZCellDive; ACC-136
TOKU-E; 2094
Anatomy/cell type resources BTO; BTO:0000665
Biological sample resources BioSample; SAMN03151730
Chemistry resources ChEMBL-Cells; CHEMBL3307959
ChEMBL-Targets; CHEMBL398
PubChem_Cell_line; CVCL_0372
Encyclopedic resources Wikidata; Q28334825
Gene expression databases GEO; GSM1374595
GEO; GSM1374596
Medical resources MeSH; D007624
Polymorphism and mutation databases Cosmic; 753567
Cosmic; 1042301
Cosmic; 1339927
Cosmic; 1969496
Cosmic; 2301581
Cosmic; 2546843
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number36