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Cellosaurus KKU-213B (CVCL_M264)

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Cell line name KKU-213B
Synonyms KKU-M214; KKUM214; M214; KKU-214; KKU214; Khon Kaen University-M214
Accession CVCL_M264
Resource Identification Initiative To cite this cell line use: KKU-213B (RRID:CVCL_M264)
Comments Problematic cell line: Contaminated. Shown to be a KKU-213A derivative (PubMed=32207095 and JCRB web site).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00592.
Population: Southeast Asian; Thai.
Doubling time: 22.68 +- 2.20 hours (PubMed=25998688); 24.5 +- 0.2 hours (PubMed=32207095).
Karyotypic information: Has lost chromosome Y.
Omics: Transcriptome analysis by microarray.
Caution: After JCRB found out that KKU-M156 and KKU-M214 were derived from KKU-M213, these three cell lines were renamed by the lab which established them: KKU-M213 became KKU-213A, KKU-M214 became KKU-M213B and KKU-M156 became KKU-M213C (PubMed=32207095).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Cys (c.37G>T); ClinVar=VCV000045123; Zygosity=Unspecified (from parent cell line).
Disease Intrahepatic cholangiocarcinoma (NCIt: C35417)
Opisthorchis viverrini-related cholangiocarcinoma (NCIt: C27769)
Cholangiocarcinoma (ORDO: Orphanet_70567)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_M261 (KKU-213A)
Children:
CVCL_C1L6 (KKU-M214/246)CVCL_C1L7 (KKU-M214/9)CVCL_HF58 (KKU-M214/GEM)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): PubMed=32207095

Markers:
AmelogeninX
CSF1PO9,13
D2S133822,23,24
D3S135815
D5S8189
D7S82011
D8S117911,13
D13S3178,12
D16S5399,11
D18S5114
D19S43313,14
D21S1129
FGA16,19
TH017
TPOX8
vWA18

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=15884115; DOI=10.3748/wjg.v11.i18.2748; PMCID=PMC4305909
Tepsiri N., Chaturat L., Sripa B., Namwat W., Wongkham S., Bhudhisawasdi V., Tassaneeyakul W.
Drug sensitivity and drug resistance profiles of human intrahepatic cholangiocarcinoma cell lines.
World J. Gastroenterol. 11:2748-2753(2005)

PubMed=16475706
Panichakul T., Intachote P., Wongkajornsilp A., Sripa B., Sirisinha S.
Triptolide sensitizes resistant cholangiocarcinoma cells to TRAIL-induced apoptosis.
Anticancer Res. 26:259-265(2006)

PubMed=18714155; DOI=10.1159/000151541
Namwat N., Amimanan P., Loilome W., Jearanaikoon P., Sripa B., Bhudhisawasdi V., Tassaneeyakul W.
Characterization of 5-fluorouracil-resistant cholangiocarcinoma cell lines.
Chemotherapy 54:343-351(2008)

PubMed=21871105; DOI=10.1186/1476-4598-10-102; PMCID=PMC3173387
Obchoei S., Weakley S.M., Wongkham S., Wongkham C., Sawanyawisuth K., Yao Q.-Z., Chen C.-Y.
Cyclophilin A enhances cell proliferation and tumor growth of liver fluke-associated cholangiocarcinoma.
Mol. Cancer 10:102.1-102.15(2011)

PubMed=22926522; DOI=10.1038/onc.2012.382; PMCID=PMC3869796
Kunkeaw N., Jeon S.H., Lee K., Johnson B.H., Tanasanvimon S., Javle M., Pairojkul C., Chamgramol Y., Wongfieng W., Gong B., Leelayuwat C., Lee Y.S.
Cell death/proliferation roles for nc886, a non-coding RNA, in the protein kinase R pathway in cholangiocarcinoma.
Oncogene 32:3722-3731(2013)

PubMed=25998688; DOI=10.3892/ijo.2015.3019
Wattanawongdon W., Hahnvajanawong C., Namwat N., Kanchanawat S., Boonmars T., Jearanaikoon P., Leelayuwat C., Techasen A., Seubwai W.
Establishment and characterization of gemcitabine-resistant human cholangiocarcinoma cell lines with multidrug resistance and enhanced invasiveness.
Int. J. Oncol. 47:398-410(2015)

PubMed=31077409; DOI=10.1002/hep.30704
Sittithumcharee G., Suppramote O., Vaeteewoottacharn K., Sirisuksakun C., Jamnongsong S., Laphanuwat P., Suntiparpluacha M., Matha A., Chusorn P., Buraphat P., Kakanaporn C., Charngkaew K., Silsirivanit A., Korphaisarn K., Limsrichamrern S., Tripatara P., Pairojkul C., Wongkham S., Sampattavanich S., Okada S., Jirawatnotai S.
Dependency of cholangiocarcinoma on cyclin D-dependent kinase activity.
Hepatology 70:1614-1630(2019)

PubMed=32207095; DOI=10.1007/s13577-020-00334-w
Sripa B., Seubwai W., Vaeteewoottacharn K., Sawanyawisuth K., Silsirivanit A., Kaewkong W., Muisuk K., Dana P., Phoomak C., Lert-itthiporn W., Luvira V., Pairojkul C., Teh B.T., Wongkham S., Okada S., Chamgramol Y.
Functional and genetic characterization of three cell lines derived from a single tumor of an Opisthorchis viverrini-associated cholangiocarcinoma patient.
Hum. Cell 33:695-708(2020)

PubMed=35640676; DOI=10.1016/j.ajpath.2022.05.007; PMCID=PMC9472155
Isidan A., Yenigun A., Soma D., Aksu E., Lopez K., Park Y., Cross-Najafi A., Li P., Kundu D., House M.G., Chakraborty S., Glaser S., Kennedy L., Francis H., Zhang W.-J., Alpini G.D., Ekser B.
Development and characterization of human primary cholangiocarcinoma cell lines.
Am. J. Pathol. 192:1200-1217(2022)

Cross-references
Cell line collections (Providers) JCRB; JCRB1556 - Discontinued
Encyclopedic resources Wikidata; Q54900011
Entry history
Entry creation05-Nov-2013
Last entry update19-Dec-2024
Version number19