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Cellosaurus EndoC-betaH2 (CVCL_IS71)

[Text version]
Cell line name EndoC-betaH2
Synonyms EndoC-BH2
Accession CVCL_IS71
Resource Identification Initiative To cite this cell line use: EndoC-betaH2 (RRID:CVCL_IS71)
Comments Characteristics: The lentiviral vector insert containing the SV40 LT and hTERT can be excised by the Cre recombinase. Following excision of immortalizing transgenes, cell proliferation sharply decreases and in parallel there is a massive enhancement of beta cell-specific features such as increased INS gene expression.
Virology: Contains an integrated xenotropic MuLV-related virus (XMRV) Bxv-1 (PubMed=26901817).
Doubling time: 5-7 days (PubMed=24667639).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Pancreas, islets of Langerhans; UBERON=UBERON_0000006.
Cell type: Pancreatic beta cell; CL=CL_0000169.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_A1TU (EndoC-betaH2-OFP-GFZ)CVCL_A1TV (EndoC-betaH21-PGF2AOF)CVCL_IS72 (EndoC-betaH3)
Sex of cell Sex unspecified
Age at sampling 9FW
Category Conditionally immortalized cell line
Web pages https://web.archive.org/web/20170502035118/http://www.endocells.com/products-and-services/
Publications

PubMed=24667639; DOI=10.1172/JCI72674; PMCID=PMC4001549
Scharfmann R., Pechberty S., Hazhouz Y., von Bulow M., Bricout-Neveu E., Grenier-Godard M., Guez F., Rachdi L., Lohmann M., Czernichow P., Ravassard P.
Development of a conditionally immortalized human pancreatic beta cell line.
J. Clin. Invest. 124:2087-2098(2014)

PubMed=26909308; DOI=10.1016/j.molmet.2015.09.008; PMCID=PMC4731729
Benazra M., Lecomte M.-J., Colace C., Muller A., Machado C., Pechberty S., Bricout-Neveu E., Grenier-Godard M., Solimena M., Scharfmann R., Czernichow P., Ravassard P.
A human beta cell line with drug inducible excision of immortalizing transgenes.
Mol. Metab. 4:916-925(2015)

PubMed=26901817; DOI=10.1172/JCI83573; PMCID=PMC4767346
Kirkegaard J.S., Ravassard P., Ingvarsen S.Z., Diedisheim M., Bricout-Neveu E., Gronborg M., Frogne T., Scharfmann R., Madsen O.D., Rescan C., Albagli-Curiel O.
Xenotropic retrovirus Bxv1 in human pancreatic beta cell lines.
J. Clin. Invest. 126:1109-1113(2016)

Cross-references
Encyclopedic resources Wikidata; Q54832323
Gene expression databases GEO; GSE48101
Entry history
Entry creation03-Mar-2017
Last entry update19-Dec-2024
Version number11