Cellosaurus EndoC-betaH2-OFP-GFZ (CVCL_A1TU)
| Cell line name | EndoC-betaH2-OFP-GFZ |
|---|---|
| Accession | CVCL_A1TU |
| Resource Identification Initiative | To cite this cell line use: EndoC-betaH2-OFP-GFZ (RRID:CVCL_A1TU) |
| Comments | Characteristics: The lentiviral vector insert containing the SV40 LT and hTERT can be excised by the Cre recombinase upon addition of tamixofen. Following excision of immortalizing transgenes, cell proliferation sharply decreases and in parallel there is a massive enhancement of beta cell-specific features such as increased INS gene expression. Characteristics: Transduced with the Fucci probe which consist of the fusion of monomeric Kusabira-Orange2 (mKO2) to the ubiquitination domain of human CDT1 (AA 30-120) and monomeric AzamiGreen (mAG) to the ubiquitination domain of human geminin (AA 1-110). Characteristics: In this cell line the Fucci probe was produced by transducing two different constructs: LTR-mKO2/CDT1-IRES-PuroR-LTR and LTR-mAG/GMNN-IRES-ZeoR-LTR. Virology: Contains an integrated xenotropic MuLV-related virus (XMRV) Bxv-1 (from parent cell line). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Met1_Thr29del and p.Ser121_Leu546del). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del). Genetic integration: Method=Transduction; Gene=Cre-ERT2 (Note=Cre recombinase fused with ligand-binding domain of ESR mutated to only bind tamoxifen). Genetic integration: Method=Transduction; Gene=FPbase; HC91W; AzamiGreen (Note=Green fluorescent protein from G.fascicularis). Genetic integration: Method=Transduction; Gene=FPbase; 134NN; mKusabira-Orange2 (Note=mKO2, orange fluorescent protein derived from F.concinna Kusabira-Orange (KO)). Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Pancreas, islets of Langerhans; UBERON=UBERON_0000006. Cell type: Pancreatic beta cell; CL=CL_0000169. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_IS71 (EndoC-betaH2) |
| Sex of cell | Sex unspecified |
| Age at sampling | 9FW |
| Category | Conditionally immortalized cell line |
| Publications | PubMed=25259951; DOI=10.1371/journal.pone.0108202; PMCID=PMC4178124 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q105506832 |
| Entry history | |
| Entry creation | 12-Jan-2021 |
| Last entry update | 19-Dec-2024 |
| Version number | 10 |