ID   EndoC-betaH2
AC   CVCL_IS71
SY   EndoC-BH2
DR   GEO; GSE48101
DR   Wikidata; Q54832323
RX   PubMed=24667639;
RX   PubMed=26901817;
RX   PubMed=26909308;
WW   https://web.archive.org/web/20170502035118/http://www.endocells.com/products-and-services/
CC   Characteristics: The lentiviral vector insert containing the SV40 LT and hTERT can be excised by the Cre recombinase. Following excision of immortalizing transgenes, cell proliferation sharply decreases and in parallel there is a massive enhancement of beta cell-specific features such as increased INS gene expression.
CC   Virology: Contains an integrated xenotropic MuLV-related virus (XMRV) Bxv-1 (PubMed=26901817).
CC   Doubling time: 5-7 days (PubMed=24667639).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Omics: Transcriptome analysis by microarray.
CC   Derived from site: In situ; Pancreas, islets of Langerhans; UBERON=UBERON_0000006.
CC   Cell type: Pancreatic beta cell; CL=CL_0000169.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Sex unspecified
AG   9FW
CA   Conditionally immortalized cell line
DT   Created: 03-03-17; Last updated: 19-12-24; Version: 11
//
RX   PubMed=24667639; DOI=10.1172/JCI72674; PMCID=PMC4001549;
RA   Scharfmann R., Pechberty S., Hazhouz Y., von Bulow M.,
RA   Bricout-Neveu E., Grenier-Godard M., Guez F., Rachdi L., Lohmann M.,
RA   Czernichow P., Ravassard P.;
RT   "Development of a conditionally immortalized human pancreatic beta
RT   cell line.";
RL   J. Clin. Invest. 124:2087-2098(2014).
//
RX   PubMed=26901817; DOI=10.1172/JCI83573; PMCID=PMC4767346;
RA   Kirkegaard J.S., Ravassard P., Ingvarsen S.Z., Diedisheim M.,
RA   Bricout-Neveu E., Gronborg M., Frogne T., Scharfmann R., Madsen O.D.,
RA   Rescan C., Albagli-Curiel O.;
RT   "Xenotropic retrovirus Bxv1 in human pancreatic beta cell lines.";
RL   J. Clin. Invest. 126:1109-1113(2016).
//
RX   PubMed=26909308; DOI=10.1016/j.molmet.2015.09.008; PMCID=PMC4731729;
RA   Benazra M., Lecomte M.-J., Colace C., Muller A., Machado C.,
RA   Pechberty S., Bricout-Neveu E., Grenier-Godard M., Solimena M.,
RA   Scharfmann R., Czernichow P., Ravassard P.;
RT   "A human beta cell line with drug inducible excision of immortalizing
RT   transgenes.";
RL   Mol. Metab. 4:916-925(2015).
//