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Cellosaurus MDCK-5KO-EKARrEV-NLS-loxP-NRG1 (CVCL_D5IS)

[Text version]
Cell line name MDCK-5KO-EKARrEV-NLS-loxP-NRG1
Accession CVCL_D5IS
Resource Identification Initiative To cite this cell line use: MDCK-5KO-EKARrEV-NLS-loxP-NRG1 (RRID:CVCL_D5IS)
Comments Characteristics: Transduced with the FRET biosensor EKARrEV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from RSK1, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal (PubMed=34667080).
Knockout cell: Method=CRISPR/Cas9; VGNC; 40235; EGF.
Knockout cell: Method=CRISPR/Cas9; VGNC; 111490; EREG.
Knockout cell: Method=CRISPR/Cas9; VGNC; 41609; HBEGF.
Knockout cell: Method=CRISPR/Cas9; VGNC; 43967; NRG1.
Knockout cell: Method=CRISPR/Cas9; VGNC; 112755; TGFA.
Genetic integration: Method=Transduction; Gene=VGNC; 43967; NRG1 (Note=With a N-terminal loxP site).
Genetic integration: Method=Transduction; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP).
Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
Hierarchy Parent: CVCL_D5IM (MDCK-4KO-EKARrEV-NLS-loxP-NRG1)
Children:
CVCL_D5J0 (MDCK-5KO-EKARrEV-NLS-loxP-NRG1-CreERT2)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=34667080; DOI=10.26508/lsa.202101206; PMCID=PMC8548211
Lin S.-H., Hirayama D., Maryu G., Matsuda K., Hino N., Deguchi E., Aoki K., Iwamoto R., Terai K., Matsuda M.
Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration.
Life Sci. Alliance 5:e202101206.1-e202101206.13(2022)

Cross-references
Encyclopedic resources Wikidata; Q127382763
Entry history
Entry creation02-May-2024
Last entry update10-Sep-2024
Version number2