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Cellosaurus MDCK-5KO-EKARrEV-NLS-loxP-NRG1-CreERT2 (CVCL_D5J0)
| Cell line name | MDCK-5KO-EKARrEV-NLS-loxP-NRG1-CreERT2 |
|---|---|
| Accession | CVCL_D5J0 |
| Resource Identification Initiative | To cite this cell line use: MDCK-5KO-EKARrEV-NLS-loxP-NRG1-CreERT2 (RRID:CVCL_D5J0) |
| Comments | Characteristics: Transduced with the FRET biosensor EKARrEV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from RSK1, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal and with CreERT2 that encodes a Cre recombinase fused to a mutant estrogen ligand-binding domain (ERT2) that requires the presence of tamoxifen for activity (PubMed=34667080). Knockout cell: Method=CRISPR/Cas9; VGNC; 40235; EGF. Knockout cell: Method=CRISPR/Cas9; VGNC; 111490; EREG. Knockout cell: Method=CRISPR/Cas9; VGNC; 41609; HBEGF. Knockout cell: Method=CRISPR/Cas9; VGNC; 43967; NRG1. Knockout cell: Method=CRISPR/Cas9; VGNC; 112755; TGFA. Genetic integration: Method=Transduction; Gene=VGNC; 43967; NRG1 (Note=With a N-terminal loxP site). Genetic integration: Method=Transduction; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP). Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Kidney; UBERON=UBERON_0002113. Cell type: Epithelial cell of kidney; CL=CL_0002518. |
| Species of origin | Canis lupus familiaris (Dog) (Canis familiaris)
(NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372. |
| Hierarchy | Parent: CVCL_D5IS (MDCK-5KO-EKARrEV-NLS-loxP-NRG1) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Transformed cell line |
| Publications | PubMed=34667080; DOI=10.26508/lsa.202101206; PMCID=PMC8548211 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q127382764 |
| Entry history | |
| Entry creation | 02-May-2024 |
| Last entry update | 10-Sep-2024 |
| Version number | 2 |