ID   MDCK-5KO-EKARrEV-NLS-loxP-NRG1
AC   CVCL_D5IS
DR   Wikidata; Q127382763
RX   PubMed=34667080;
CC   Characteristics: Transduced with the FRET biosensor EKARrEV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from RSK1, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal (PubMed=34667080).
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; 40235; EGF.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; 111490; EREG.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; 41609; HBEGF.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; 43967; NRG1.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; 112755; TGFA.
CC   Genetic integration: Method=Transduction; Gene=VGNC; 43967; NRG1 (Note=With a N-terminal loxP site).
CC   Genetic integration: Method=Transduction; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP).
CC   Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
CC   Cell type: Epithelial cell of kidney; CL=CL_0002518.
CC   Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
OX   NCBI_TaxID=9615; ! Canis lupus familiaris (Dog)
HI   CVCL_D5IM ! MDCK-4KO-EKARrEV-NLS-loxP-NRG1
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 02-05-24; Last updated: 10-09-24; Version: 2
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RX   PubMed=34667080; DOI=10.26508/lsa.202101206; PMCID=PMC8548211;
RA   Lin S.-H., Hirayama D., Maryu G., Matsuda K., Hino N., Deguchi E.,
RA   Aoki K., Iwamoto R., Terai K., Matsuda M.;
RT   "Redundant roles of EGFR ligands in the ERK activation waves during
RT   collective cell migration.";
RL   Life Sci. Alliance 5:e202101206.1-e202101206.13(2022).
//