Cellosaurus cell line MDCK-4KO-EKARrEV-NLS-loxP-NRG1 (CVCL

Cellosaurus MDCK-4KO-EKARrEV-NLS-loxP-NRG1 (CVCL_D5IM)

[Text version]

Cell line name

MDCK-4KO-EKARrEV-NLS-loxP-NRG1

Accession

CVCL_D5IM

Resource Identification Initiative

To cite this cell line use: MDCK-4KO-EKARrEV-NLS-loxP-NRG1 (RRID:CVCL_D5IM)

Comments

Characteristics: Transduced with the FRET biosensor EKARrEV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from RSK1, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal (PubMed=34667080).
Knockout cell: Method=CRISPR/Cas9; VGNC; 40235; EGF.
Knockout cell: Method=CRISPR/Cas9; VGNC; 111490; EREG.
Knockout cell: Method=CRISPR/Cas9; VGNC; 41609; HBEGF.
Knockout cell: Method=CRISPR/Cas9; VGNC; 112755; TGFA.
Genetic integration: Method=Transduction; Gene=VGNC; 43967; NRG1 (Note=With a N-terminal loxP site).
Genetic integration: Method=Transduction; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP).
Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.

Species of origin

Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.

Hierarchy

Parent: CVCL_D5IH (MDCK-4KO-EKARrEV-NLS)
Children:

CVCL_D5IS (MDCK-5KO-EKARrEV-NLS-loxP-NRG1)

Sex of cell

Female

Age at sampling

Adult