| Abstract |
The largest U.S. cancer health disparity exists in prostate cancer, with
African American men having the highest incidence and mortality rates. The
present study evaluated the effects of ZIC2 and the underlying mechanisms
in the E006 parental African-American cell line that produces tumors at
accelerated growth rates because of the increase of ZIC2 genes in African-
American males. We analyzed the experimental research that the
overexpression of ZIC2 contributes to progression of prostate cancer.
E006AA cells with overexpressed or suppressed ZIC2 were analyzed to
determine phenotypic differences, PCR, cell proliferation and immunoblot
assays. The expression levels of ZIC2 were analyzed by CRISPR-Cas9,
Western blot and proliferation growth curves. We discovered using these
experimental techniques to knockout ZIC2, reduced cell proliferation
occurred. This research investigated the role of ZIC2 in prostate cancer
progression and the effects of the loss or gain of function of ZIC2 by
using CRISPR-Cas 9 genome editing technology.
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