Cellosaurus cell line U2OS MPX-Nomad (CVCL

Cellosaurus U2OS MPX-Nomad (CVCL_YK69)

[Text version]

Cell line name

U2OS MPX-Nomad

Synonyms

MPXNomad; MultiPleXed-Nomad U2OS

Accession

CVCL_YK69

Resource Identification Initiative

To cite this cell line use: U2OS MPX-Nomad (RRID:CVCL_YK69)

Comments

Population: Caucasian.
Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal.
Characteristics: The red cAMP-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboFP650 fluorescent protein, a Ht31 peptide fused to a KDEL signal.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; JS838; TurboFP650 (Note=Far-red fluorescent protein derived from eqFP578).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; A315M; TurboGFP (Note=Green fluorescent protein from P.plumata).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.

Sequence variations

Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).

Disease

Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0042 (U2OS)
Children:

CVCL_YK70 (U2OS MPX-Nomad ETBR)

CVCL_YK71 (U2OS MPX-Nomad NK2R)

Sex of cell

Female

Age at sampling

15Y