Cellosaurus logo
expasy logo

Cellosaurus U2OS MPX-Nomad NK2R (CVCL_YK71)

[Text version]
Cell line name U2OS MPX-Nomad NK2R
Synonyms MPXNomad NK2R; MultiPleXed-Nomad NK2R U2OS
Accession CVCL_YK71
Resource Identification Initiative To cite this cell line use: U2OS MPX-Nomad NK2R (RRID:CVCL_YK71)
Comments Population: Caucasian.
Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal.
Characteristics: The red cAMP-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboFP650 fluorescent protein, a Ht31 peptide fused to a KDEL signal.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:11527; TACR2.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; JS838; TurboFP650 (Note=Far-red fluorescent protein derived from eqFP578).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; A315M; TurboGFP (Note=Green fluorescent protein from P.plumata).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_YK69 (U2OS MPX-Nomad)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=29412765; DOI=10.1177/2472630318754828
Mella R.M., Kortazar D., Roura-Ferrer M., Salado C., Valcarcel M., Castilla A., Villace P.
Nomad biosensors: a new multiplexed technology for the screening of GPCR ligands.
SLAS Technol. 23:207-216(2018)

Cross-references
Encyclopedic resources Wikidata; Q98133759
Entry history
Entry creation19-Dec-2019
Last entry update19-Dec-2024
Version number11