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Cellosaurus CF8Flp (CVCL_WZ18)

[Text version]
Cell line name CF8Flp
Accession CVCL_WZ18
Resource Identification Initiative To cite this cell line use: CF8Flp (RRID:CVCL_WZ18)
Comments Characteristics: Contains a single recombination Flp-in target site for the stable integration and expression of a single cDNA, mini-gene, or complete gene.
Characteristics: Transfected with the pFRT/lacZeo plasmid. A single Flp recombination target (FRT) was integrated into chromosome 8.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSVori-).
Derived from site: In situ; Lung, bronchus, epithelium; UBERON=UBERON_0002031.
Cell type: Epithelial cell of bronchus; CL=CL_0002328.
Sequence variations
  • Mutation; HGNC; HGNC:1884; CFTR; Simple; p.Phe508del (c.1521_1523delCTT); ClinVar=VCV000007105; Zygosity=Homozygous (from parent cell line).
Disease Cystic fibrosis (NCIt: C2975)
Cystic fibrosis (ORDO: Orphanet_586)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_HL93 (CFBE41o-)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Transformed cell line
Web pages http://jhu.technologypublisher.com/technology/23388
Publications

PubMed=26694805; DOI=10.1016/j.jcf.2015.11.010; PMCID=PMC4879073
Gottschalk L.B., Vecchio-Pagan B., Sharma N., Han S.T., Franca A., Wohler E.S., Batista D.A.S., Goff L.A., Cutting G.R.
Creation and characterization of an airway epithelial cell line for stable expression of CFTR variants.
J. Cyst. Fibros. 15:285-294(2016)

Cross-references
Encyclopedic resources Wikidata; Q93447351
Entry history
Entry creation06-Sep-2019
Last entry update19-Dec-2024
Version number11