ID   CF8Flp
AC   CVCL_WZ18
DR   Wikidata; Q93447351
RX   PubMed=26694805;
WW   http://jhu.technologypublisher.com/technology/23388
CC   Characteristics: Contains a single recombination Flp-in target site for the stable integration and expression of a single cDNA, mini-gene, or complete gene.
CC   Characteristics: Transfected with the pFRT/lacZeo plasmid. A single Flp recombination target (FRT) was integrated into chromosome 8.
CC   Sequence variation: Mutation; HGNC; HGNC:1884; CFTR; Simple; p.Phe508del (c.1521_1523delCTT); ClinVar=VCV000007105; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSVori-).
CC   Derived from site: In situ; Lung, bronchus, epithelium; UBERON=UBERON_0002031.
CC   Cell type: Epithelial cell of bronchus; CL=CL_0002328.
DI   NCIt; C2975; Cystic fibrosis
DI   ORDO; Orphanet_586; Cystic fibrosis
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_HL93 ! CFBE41o-
SX   Sex unspecified
AG   Age unspecified
CA   Transformed cell line
DT   Created: 06-09-19; Last updated: 19-12-24; Version: 11
//
RX   PubMed=26694805; DOI=10.1016/j.jcf.2015.11.010; PMCID=PMC4879073;
RA   Gottschalk L.B., Vecchio-Pagan B., Sharma N., Han S.T., Franca A.,
RA   Wohler E.S., Batista D.A.S., Goff L.A., Cutting G.R.;
RT   "Creation and characterization of an airway epithelial cell line for
RT   stable expression of CFTR variants.";
RL   J. Cyst. Fibros. 15:285-294(2016).
//