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Cellosaurus HTC75 (CVCL_WM79)

[Text version]
Cell line name HTC75
Accession CVCL_WM79
Resource Identification Initiative To cite this cell line use: HTC75 (RRID:CVCL_WM79)
Comments Population: Caucasian.
Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0317 (HT-1080)
Children:
CVCL_WM80 (HTC75 T19)CVCL_WM81 (HTC75 T4)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line
STR profile Source(s): PubMed=19864690

Markers:
CSF1PO12
D2S133825,26
D3S135816
D5S81811,13
D8S117913,14
D13S31712,14
D16S5399,12
D18S5112,18
D19S43313.2,15
D21S1128,30
FGA22,25
TH016
TPOX8
vWA14,19

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Publications

PubMed=9034193; DOI=10.1038/385740a0
van Steensel B., de Lange T.
Control of telomere length by the human telomeric protein TRF1.
Nature 385:740-743(1997)

PubMed=9476899; DOI=10.1016/S0092-8674(00)80932-0
van Steensel B., Smogorzewska A., de Lange T.
TRF2 protects human telomeres from end-to-end fusions.
Cell 92:401-413(1998)

PubMed=19864690; DOI=10.1074/jbc.M109.038026; PMCID=PMC2801271
Takai K.K., Hooper S., Blackwood S., Gandhi R., de Lange T.
In vivo stoichiometry of shelterin components.
J. Biol. Chem. 285:1457-1467(2010)

Cross-references
Cell line databases/resources cancercelllines; CVCL_WM79
Encyclopedic resources Wikidata; Q94305579
Experimental variables resources EFO; EFO_0022531
Entry history
Entry creation24-May-2019
Last entry update19-Dec-2024
Version number13