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Cellosaurus HTC75 T4 (CVCL_WM81)

[Text version]
Cell line name HTC75 T4
Synonyms HTC75T4; HTC75 clone T4; T4
Accession CVCL_WM81
Resource Identification Initiative To cite this cell line use: HTC75 T4 (RRID:CVCL_WM81)
Comments Population: Caucasian.
Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:11729; TERF2 (Note=With p.Met1_Ala44del and p.Lys501_Asn542del and a N-terminal FLAG tag = TRF2deltaBdeltaM).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_WM79 (HTC75)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line
Publications

PubMed=9476899; DOI=10.1016/S0092-8674(00)80932-0
van Steensel B., Smogorzewska A., de Lange T.
TRF2 protects human telomeres from end-to-end fusions.
Cell 92:401-413(1998)

Cross-references
Cell line databases/resources cancercelllines; CVCL_WM81
Encyclopedic resources Wikidata; Q94305591
Entry history
Entry creation24-May-2019
Last entry update19-Dec-2024
Version number15