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Cellosaurus LFBK-alphaVbeta6 (CVCL_RX27)

[Text version]
Cell line name LFBK-alphaVbeta6
Synonyms LFBK alphaVbeta6; LFBKalphaVbeta6; LF-BK alphaVbeta6; LFBK-alpha(v)beta(6); LFBK-aVb6; LFBK-AvB6; LFBKaVB6; LFBKavb6
Accession CVCL_RX27
Resource Identification Initiative To cite this cell line use: LFBK-alphaVbeta6 (RRID:CVCL_RX27)
Comments Group: Patented cell line.
Problematic cell line: Misidentified. Parent cell line (LF-BK) was originally thought to be of bovine fetal kidney origin but originates from pig (PubMed=25617444). The derived cell line has also been shown to originate from pig (PubMed=34737324).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00613.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-13047.
Virology: More susceptible to infection by foot-and-mouth disease virus than parent cell line.
Virology: Susceptible to infection by senecavirus A (SVA) which can be used as a surrogate for studies of goot-and-mouth disease virus (PubMed=36146682).
Virology: Persistently infected by bovine viral diarrhea virus (BVDV) (PubMed=34092457; PubMed=34458357; PubMed=35112907).
Genetic integration: Method=Transfection/transduction; Gene=VGNC; 30323; Bovine ITGAV.
Genetic integration: Method=Transfection/transduction; Gene=VGNC; 30331; Bovine ITGB6.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Hierarchy Parent: CVCL_RX26 (LF-BK)
Children:
CVCL_A0GW (MGPK alphaVbeta6-C5)CVCL_C4XX (WRL-LFBK-alphavbeta6)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Spontaneously immortalized cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=23515553; DOI=10.1128/jcm.03370-12; PMCID=PMC3716081
LaRocco M.A., Krug P.W., Kramer E., Ahmed Z., Pacheco J.M., Duque H., Baxt B., Rodriguez L.L.
A continuous bovine kidney cell line constitutively expressing bovine alphavbeta6 integrin has increased susceptibility to foot-and-mouth disease virus.
J. Clin. Microbiol. 51:1714-1720(2013)

Patent=US9121010
LaRocco M.A., Baxt B., Duque H.
Continuous porcine kidney cell line constitutively expressing bovine alphaVbeta6 integrin with increased susceptibility to foot and mouth disease virus.
Patent number US9121010, 01-Sep-2015

PubMed=25617444; DOI=10.1128/jcm.03220-14; PMCID=PMC4298512
LaRocco M.A., Krug P.W., Kramer E., Ahmed Z., Pacheco J.M., Duque H., Baxt B., Rodriguez L.L.
Correction: A continuous bovine kidney cell line constitutively expressing bovine alphaVbeta6 integrin has increased susceptibility to foot-and-mouth disease virus.
J. Clin. Microbiol. 53:755-755(2015)

PubMed=26082457; DOI=10.1177/1040638715584156
Fukai K., Morioka K., Yamada M., Nishi T., Yoshida K., Kitano R., Yamazoe R., Kanno T.
Comparative performance of fetal goat tongue cell line ZZ-R 127 and fetal porcine kidney cell line LFBK-alphavbeta6 for foot-and-mouth disease virus isolation.
J. Vet. Diagn. Invest. 27:516-521(2015)

PubMed=32851014; DOI=10.3389/fvets.2020.00426; PMCID=PMC7401924
Gray A.R., Wood B.A., Henry E., Azhar M., King D.P., Mioulet V.
Evaluation of cell lines for the isolation of foot-and-mouth disease virus and other viruses causing vesicular disease.
Front. Vet. Sci. 7:426.1-426.11(2020)

PubMed=34092457; DOI=10.1016/j.biologicals.2021.05.003
LaRocco M.A., Ahmed Z., Rodriguez-Calzada M., Azzinaro P.A., Barrette R.W., Krug P.W., Rodriguez L.L., de Los Santos T., Medina G.N.
An adventitious agent-free clonal cell line that is highly susceptible to foot -and-mouth disease virus.
Biologicals 72:33-41(2021)

PubMed=34458357; DOI=10.3389/fvets.2021.715120; PMCID=PMC8385193
Gray A.R., Wood B.A., Henry E., King D.P., Mioulet V.
Elimination of non-cytopathic bovine viral diarrhea virus from the LFBK-alphavbeta6 cell line.
Front. Vet. Sci. 8:715120.1-715120.5(2021)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5; PMCID=PMC8569163
Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657-21657(2021)

PubMed=35112907; DOI=10.1128/mra.01167-21; PMCID=PMC8812309
Holinka-Patterson L.G., Fish I.H., Bertram M.R., Hartwig E.J., Smoliga G.R., Stenfeldt C., Rodriguez L.L., Arzt J.
Genome of bovine viral diarrhea virus (BVDV) contaminating a continuous LFBK-alphaVbeta6 cell line.
Microbiol. Resour. Announc. 11:e01167.21.1-e01167.21.2(2022)

PubMed=36146682; DOI=10.3390/v14091875; PMCID=PMC9503874
Mason J., Primavera V., Martignette L., Clark B., Barrera J., Simmons J., Hurtle W., Neilan J.G., Puckette M.
Comparative evaluation of the foot-and-mouth disease virus permissive LF-BK alphaVbeta6 cell line for senecavirus A research.
Viruses 14:1875.1-1875.12(2022)

Cross-references
Encyclopedic resources Wikidata; Q54902456
Entry history
Entry creation05-Mar-2018
Last entry update10-Sep-2024
Version number11