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Cellosaurus EndoC-betaH1 (CVCL_L909)

[Text version]
Cell line name EndoC-betaH1
Synonyms EndoC-BH1
Accession CVCL_L909
Resource Identification Initiative To cite this cell line use: EndoC-betaH1 (RRID:CVCL_L909)
Comments Characteristics: Secretes insulin in response to glucose challenge.
Virology: Contains an integrated xenotropic MuLV-related virus (XMRV) Bxv-1 (PubMed=26901817).
Doubling time: 5 days (PubMed=21865645); 174 hours (PubMed=25803449).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Omics: H3K27ac ChIP-seq epigenome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K36me3 ChIP-seq epigenome analysis.
Omics: H3K4me1 ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K79me2 ChIP-seq epigenome analysis.
Omics: H3K9me2 ChIP-seq epigenome analysis.
Omics: H3K9me3 ChIP-seq epigenome analysis.
Omics: miRNA expression profiling.
Omics: Transcriptome analysis by RNAseq.
Miscellaneous: Sex assignment derived from omics analysis.
Derived from site: In situ; Pancreas, islets of Langerhans; UBERON=UBERON_0000006.
Cell type: Pancreatic beta cell; CL=CL_0000169.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_YB06 (EndoC-betaH1 IDE-KO)CVCL_YB07 (EndoC-betaH1 INS-KO)CVCL_YB08 (EndoC-betaH1 NEUROD1-KO)
CVCL_YB09 (EndoC-betaH1 PAM-KO)CVCL_YB10 (EndoC-betaH1 SLC30A8-KO)
Sex of cell Female
Age at sampling 7-11FW
Category Transformed cell line
Web pages https://web.archive.org/web/20170502035118/http://www.endocells.com/products-and-services/
https://www.humancelldesign.com/human-beta-cells-endoc-bh1/
Publications

PubMed=21865645; DOI=10.1172/JCI58447; PMCID=PMC3163974
Ravassard P., Hazhouz Y., Pechberty S., Bricout-Neveu E., Armanet M., Czernichow P., Scharfmann R.
A genetically engineered human pancreatic beta cell line exhibiting glucose-inducible insulin secretion.
J. Clin. Invest. 121:3589-3597(2011)

PubMed=21865653; DOI=10.1172/JCI58899; PMCID=PMC3163975
Weir G.C., Bonner-Weir S.
Finally! A human pancreatic beta cell line.
J. Clin. Invest. 121:3395-3397(2011)

PubMed=25803449; DOI=10.1371/journal.pone.0120879; PMCID=PMC4372368
Andersson L.E., Valtat B., Bagge A., Sharoyko V.V., Nicholls D.G., Ravassard P., Scharfmann R., Spegel P., Mulder H.
Characterization of stimulus-secretion coupling in the human pancreatic EndoC-betaH1 beta cell line.
PLoS ONE 10:E0120879-E0120879(2015)

PubMed=25822155; DOI=10.1097/MPA.0000000000000318
Krishnan K., Ma Z.-H., Bjorklund A., Islam M.S.
Calcium signaling in a genetically engineered human pancreatic beta-cell line.
Pancreas 44:773-777(2015)

PubMed=26901817; DOI=10.1172/JCI83573; PMCID=PMC4767346
Kirkegaard J.S., Ravassard P., Ingvarsen S.Z., Diedisheim M., Bricout-Neveu E., Gronborg M., Frogne T., Scharfmann R., Madsen O.D., Rescan C., Albagli-Curiel O.
Xenotropic retrovirus Bxv1 in human pancreatic beta cell lines.
J. Clin. Invest. 126:1109-1113(2016)

PubMed=29307512; DOI=10.1016/j.molmet.2017.12.007; PMCID=PMC5985049
Tsonkova V.G., Sand F.W., Wolf X.A., Grunnet L.G., Ringgaard A.K., Ingvorsen C., Winkel L., Kalisz M., Dalgaard K., Bruun C., Fels J.J., Helgstrand C., Hastrup S., Oberg F.K., Vernet E., Sandrini M.P.B., Shaw A.C., Jessen C., Gronborg M., Hald J., Willenbrock H., Madsen D., Wernersson R., Hansson L., Jensen J.N., Plesner A., Alanentalo T., Petersen M.B.K., Grapin-Botton A., Honore C., Ahnfelt-Ronne J., Hecksher-Sorensen J., Ravassard P., Madsen O.D., Rescan C., Frogne T.
The EndoC-betaH1 cell line is a valid model of human beta cells and applicable for screenings to identify novel drug target candidates.
Mol. Metab. 8:144-157(2018)

PubMed=30251567; DOI=10.1177/0963689718798564; PMCID=PMC6180725
Pellegrini S., Manenti F., Chimienti R., Nano R., Ottoboni L., Ruffini F., Martino G., Ravassard P., Piemonti L., Sordi V.
Differentiation of Sendai virus-reprogrammed iPSC into beta cells, compared with human pancreatic islets and immortalized beta cell line.
Cell Transplant. 27:1548-1560(2018)

PubMed=31976379; DOI=10.12688/wellcomeopenres.15447.2; PMCID=PMC6961417
Grotz A.K., Abaitua F., Navarro-Guerrero E., Hastoy B., Ebner D., Gloyn A.L.
A CRISPR/Cas9 genome editing pipeline in the EndoC-betaH1 cell line to study genes implicated in beta cell function.
Wellcome Open Res. 4:150-150(2019)

Cross-references
Encyclopedic resources Wikidata; Q54832322
Gene expression databases GEO; GSE118588
Proteomic databases PRIDE; PXD027920
Entry history
Entry creation06-May-2013
Last entry update19-Dec-2024
Version number23