Cellosaurus HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I TMEM192-3xHA (CVCL_E4JW)
| Cell line name | HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I TMEM192-3xHA |
|---|---|
| Synonyms | Syn10 RNF152 reporter with lyso-tag |
| Accession | CVCL_E4JW |
| Resource Identification Initiative | To cite this cell line use: HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I TMEM192-3xHA (RRID:CVCL_E4JW) |
| Comments | Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). Characteristics: Additional expression of a plasmid encoding a bicistronic IRES construct of EGFP-tagged RNF152, along with an mScarlet-I transfection marker (from parent cell line). Characteristics: Additional transduction with a construct encoding for the LysoTag, the lysosomal protein TMEM192 tagged with 3xHA (Direct_author_submission). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:26811; RNF152. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11138; SNCA (Note=With p.Ala53Thr). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:26775; TMEM192 (Note=With a 3xHA tag). Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=FPbase; 6VVTK; mScarlet-I (Note=Red fluorescent protein). Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen. Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA). Miscellaneous: Cell line information from personal communication of Yuste-Checa, Patricia. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_D4BY (HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Entry history | |
| Entry creation | 19-Dec-2024 |
| Last entry update | 19-Dec-2024 |
| Version number | 1 |