ID   HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I TMEM192-3xHA
AC   CVCL_E4JW
SY   Syn10 RNF152 reporter with lyso-tag
CC   Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line).
CC   Characteristics: Additional expression of a plasmid encoding a bicistronic IRES construct of EGFP-tagged RNF152, along with an mScarlet-I transfection marker (from parent cell line).
CC   Characteristics: Additional transduction with a construct encoding for the LysoTag, the lysosomal protein TMEM192 tagged with 3xHA (Direct_author_submission).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:26811; RNF152.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11138; SNCA (Note=With p.Ala53Thr).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:26775; TMEM192 (Note=With a 3xHA tag).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=FPbase; 6VVTK; mScarlet-I (Note=Red fluorescent protein).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Miscellaneous: Cell line information from personal communication of Yuste-Checa, Patricia.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D4BY ! HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 19-12-24; Last updated: 19-12-24; Version: 1
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