Cellosaurus HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I (CVCL_D4BY)
Cell line name | HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I |
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Synonyms | Syn10 RNF152 reporter |
Accession | CVCL_D4BY |
Resource Identification Initiative | To cite this cell line use: HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I (RRID:CVCL_D4BY) |
Comments | Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). Characteristics: Additional expression of a plasmid encoding a bicistronic IRES construct of EGFP-tagged RNF152, along with an mScarlet-I transfection marker (Direct_author_submission). Transfected with: HGNC; 26811; RNF152. Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: mScarlet, a red fluorescent protein. Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA]. Miscellaneous: Cell line information from personal communication of Yuste-Checa P. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_D4BW (HEK293T TetOff-SNCA-A53T) |
Sex of cell | Female |
Age at sampling | Fetus |
Category | Transformed cell line |
Entry history | |
Entry creation | 30-Jan-2024 |
Last entry update | 30-Jan-2024 |
Version number | 1 |