Cellosaurus UMANe002-A-3 (CVCL_E3RR)
| Cell line name | UMANe002-A-3 |
|---|---|
| Synonyms | BIRe003-A-3 |
| Accession | CVCL_E3RR |
| Resource Identification Initiative | To cite this cell line use: UMANe002-A-3 (RRID:CVCL_E3RR) |
| Comments | From: University of Manchester; Manchester; United Kingdom. Population: Caucasian. Characteristics: Produces a robust fluorescent signal in cardiac cells and have wide utility particularly for research on developmental biology and disease modelling (from parent cell line). Characteristics: Using CRISPR/Cas9 a T2A-dTomato construct was integrated at the 3'end of both alleles of HAND1 (PubMed=38460234). Characteristics: Using CRISPR/Cas9 a T2A-eGFP construct was integrated at the 3'end of one allele of NKX2-5 (from parent cell line). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_D6JR (UMANe002-A-2) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=38460234; DOI=10.1016/j.scr.2024.103342 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; UMANe002-A-3 |
| Biological sample resources | BioSamples; SAMEA114526381 |
| Entry history | |
| Entry creation | 19-Dec-2024 |
| Last entry update | 19-Dec-2024 |
| Version number | 1 |