ID   UMANe002-A-3
AC   CVCL_E3RR
SY   BIRe003-A-3
DR   BioSamples; SAMEA114526381
DR   hPSCreg; UMANe002-A-3
RX   PubMed=38460234;
CC   From: University of Manchester; Manchester; United Kingdom.
CC   Population: Caucasian.
CC   Characteristics: Produces a robust fluorescent signal in cardiac cells and have wide utility particularly for research on developmental biology and disease modelling (from parent cell line).
CC   Characteristics: Using CRISPR/Cas9 a T2A-dTomato construct was integrated at the 3'end of both alleles of HAND1 (PubMed=38460234).
CC   Characteristics: Using CRISPR/Cas9 a T2A-eGFP construct was integrated at the 3'end of one allele of NKX2-5 (from parent cell line).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D6JR ! UMANe002-A-2
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 19-12-24; Last updated: 19-12-24; Version: 1
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RX   PubMed=38460234; DOI=10.1016/j.scr.2024.103342;
RA   Lynch A.T., Douglas M., Kimber S.J., Birket M.J.;
RT   "The generation and validation of a dual cardiac HAND1-Tomato
RT   NKX2-5-GFP human embryonic stem cell line UMANe002-A-3.";
RL   Stem Cell Res. 77:103342-103342(2024).
//