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Cellosaurus UMANe002-A-2 (CVCL_D6JR)

[Text version]
Cell line name UMANe002-A-2
Accession CVCL_D6JR
Resource Identification Initiative To cite this cell line use: UMANe002-A-2 (RRID:CVCL_D6JR)
Comments Characteristics: Produces a robust fluorescent signal in cardiac cells and have wide utility particularly for research on developmental biology and disease modelling (PubMed=38100908).
Characteristics: Using CRISPR/Cas9 a T2A-eGFP construct was integrated at the 3'end of one allele of NKX2-5 (PubMed=38100908).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_L201 (Man-13)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=38100908; DOI=10.1016/j.scr.2023.103262
Douglas M., O'Loughlin C., Lynch A.T., Prialgauskaite R., Adamson A.D., Dibb K.M., Kimber S.J., Birket M.J.
The generation and validation of two NKX2-5 fluorescent reporter human embryonic stem cell lines: UMANe002-A-1 and UMANe002-A-2.
Stem Cell Res. 74:103262-103262(2024)

Cross-references
Cell line databases/resources hPSCreg; UMANe002-A-2
Biological sample resources BioSamples; SAMEA114295974
Encyclopedic resources Wikidata; Q127384582
Entry history
Entry creation02-May-2024
Last entry update10-Sep-2024
Version number2