Cellosaurus logo
expasy logo

Cellosaurus HEK293 TC4-2A (CVCL_E3BV)

[Text version]
Cell line name HEK293 TC4-2A
Synonyms TC4-2A
Accession CVCL_E3BV
Resource Identification Initiative To cite this cell line use: HEK293 TC4-2A (RRID:CVCL_E3BV)
Comments Characteristics: Using pcDNA5/FRT/TO and pOG44, a circRNA encompassing exons 2A, 2B, 3 and 4 of human SMN gene was integrated in a single site and its expression is regulated by doxycycline (PubMed=35885927).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 11117; SMN1 (Note=circRNA with exons 2A, 2B, 3 and 4).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Omics: Deep quantitative proteome analysis.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D585 (T-REx-293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=35885927; DOI=10.3390/genes13071145; PMCID=PMC9323214
Luo D., Singh N.N., Singh R.N.
Internal introns promote backsplicing to generate circular RNAs from spinal muscular atrophy gene.
Genes (Basel) 13:1145.1-1145.20(2022)

PubMed=38714739; DOI=10.1038/s41598-024-60593-7; PMCID=PMC11076517
Luo D., Ottesen E.W., Lee J.H., Singh R.N.
Transcriptome- and proteome-wide effects of a circular RNA encompassing four early exons of the spinal muscular atrophy genes.
Sci. Rep. 14:10442-10442(2024)

Entry history
Entry creation10-Sep-2024
Last entry update10-Sep-2024
Version number1