ID   HEK293 TC4-2A
AC   CVCL_E3BV
SY   TC4-2A
RX   PubMed=35885927;
RX   PubMed=38714739;
CC   Characteristics: Using pcDNA5/FRT/TO and pOG44, a circRNA encompassing exons 2A, 2B, 3 and 4 of human SMN gene was integrated in a single site and its expression is regulated by doxycycline (PubMed=35885927).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; 11117; SMN1 (Note=circRNA with exons 2A, 2B, 3 and 4).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Omics: Deep quantitative proteome analysis.
CC   Omics: Transcriptome analysis by RNAseq.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D585 ! T-REx-293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 10-09-24; Last updated: 10-09-24; Version: 1
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RX   PubMed=35885927; DOI=10.3390/genes13071145; PMCID=PMC9323214;
RA   Luo D., Singh N.N., Singh R.N.;
RT   "Internal introns promote backsplicing to generate circular RNAs from
RT   spinal muscular atrophy gene.";
RL   Genes (Basel) 13:1145.1-1145.20(2022).
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RX   PubMed=38714739; DOI=10.1038/s41598-024-60593-7; PMCID=PMC11076517;
RA   Luo D., Ottesen E.W., Lee J.H., Singh R.N.;
RT   "Transcriptome- and proteome-wide effects of a circular RNA
RT   encompassing four early exons of the spinal muscular atrophy genes.";
RL   Sci. Rep. 14:10442-10442(2024).
//